Data on mitochondrial respiratory function in skeletal muscle of adult male mice in response to 3-weeks heat stress

Rikuhide Koma; Tatsuki Matsumoto; Ayaka Yamazaki; Tsubasa Shibaguchi; Thomas Jue; Kazumi Masuda

doi:10.1016/j.dib.2024.110091

Data on mitochondrial respiratory function in skeletal muscle of adult male mice in response to 3-weeks heat stress

Data Brief. 2024 Jan 24:53:110091. doi: 10.1016/j.dib.2024.110091. eCollection 2024 Apr.

Authors

Rikuhide Koma^{1

2}, Tatsuki Matsumoto³, Ayaka Yamazaki⁴, Tsubasa Shibaguchi⁵, Thomas Jue⁶, Kazumi Masuda⁷

Affiliations

¹ Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa, 920-1192, Japan.
² Research Fellowship for Young Scientists, Japan Society for the Promotion of Science, Tokyo, 102-0083, Japan.
³ School of Teacher Education, Kanazawa University, Ishikawa, 920-1192, Japan.
⁴ Graduate School of Frontier Science Initiative, Kanazawa University, Ishikawa, 920-1192, Japan.
⁵ Institute of Liberal Arts and Science, Kanazawa University, Ishikawa, 920-1192, Japan.
⁶ Department of Biochemistry and Molecular Medicine, University of California Davis, Davis, 95616-8635, USA.
⁷ Institute of Human and Social Sciences, Kanazawa University, Ishikawa, 920-1192, Japan.

Abstract

Chronic heat stress induces mitochondrial adaptation in skeletal muscle. However, the effect of chronic heat stress on the respiratory function per mitochondria in skeletal muscle has not been well studied. Here, the present study reports on the effect of 3-weeks heat stress on muscle mitochondrial respiration using male C57BL/6JJ mice at age 21 weeks. Mice were randomly assigned to either the control group (n = 6) or passive heat group (n = 6). After 3-weeks of heat stress, the right triceps surae was removed and used for biochemical analysis. Protein expression was assessed by immunoblotting. Mitochondrial respiratory function was measured by Oxygraph-2k. The study also shows the impact of the heat stress on daily feeding, body weight, muscle weight, and protein expression of heat shock proteins (heat-response marker).

Keywords: Isolated mitochondria; Mitochondrial oxygen consumption; Oxygraph-2k; Triceps surae.