Tomato CYP94C1 inactivates bioactive JA-Ile to attenuate jasmonate-mediated defense during fruit ripening

Tianxia Yang; Lei Deng; Qinyang Wang; Chuanlong Sun; Muhammad Ali; Fangming Wu; Huawei Zhai; Qian Xu; Peiyong Xin; Shujing Cheng; Jinfang Chu; Tingting Huang; Chang-Bao Li; Chuanyou Li

doi:10.1016/j.molp.2024.02.004

Tomato CYP94C1 inactivates bioactive JA-Ile to attenuate jasmonate-mediated defense during fruit ripening

Mol Plant. 2024 Apr 1;17(4):509-512. doi: 10.1016/j.molp.2024.02.004. Epub 2024 Feb 7.

Authors

Tianxia Yang¹, Lei Deng², Qinyang Wang³, Chuanlong Sun⁴, Muhammad Ali¹, Fangming Wu³, Huawei Zhai⁵, Qian Xu⁶, Peiyong Xin¹, Shujing Cheng¹, Jinfang Chu³, Tingting Huang⁷, Chang-Bao Li⁸, Chuanyou Li⁹

Affiliations

¹ Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
² Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China; Taishan Academy of Tomato Innovation, Tai'an 271018, China. Electronic address: ldeng@genetics.ac.cn.
³ Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Taishan Academy of Tomato Innovation, Tai'an 271018, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
⁵ Taishan Academy of Tomato Innovation, Tai'an 271018, China; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
⁶ College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
⁷ Institute of Vegetable, Qingdao Academy of Agricultural Sciences, Qingdao 266100, China.
⁸ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
⁹ Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China; Taishan Academy of Tomato Innovation, Tai'an 271018, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: cyli@genetics.ac.cn.

PMID: 38327053
DOI: 10.1016/j.molp.2024.02.004

Abstract

As the master regulators of the ET signaling pathway, EIL transcription factors directly activate the expression of CYP94C1 to inactivate bioactive JA-Ile, thereby attenuating JA-mediated defense during fruit ripening. Knockout of CYP94C1 improves tomato fruit resistance to necrotrophs without compromising fruit quality.

MeSH terms

Cyclopentanes / metabolism
Fruit / genetics
Fruit / metabolism
Gene Expression Regulation, Plant
Isoleucine / analogs & derivatives*
Oxylipins / metabolism
Solanum lycopersicum* / genetics

Substances

jasmonic acid
jasmonoyl-isoleucine
Oxylipins
Cyclopentanes
Isoleucine