Rab11 promotes single Mauthner cell axon regeneration in vivo through axon guidance molecule Ntng2b

Huaitong Yao; Yueru Shen; Zheng Song; Along Han; Xinghan Chen; Yawen Zhang; Bing Hu

doi:10.1016/j.expneurol.2024.114715

Rab11 promotes single Mauthner cell axon regeneration in vivo through axon guidance molecule Ntng2b

Exp Neurol. 2024 Apr:374:114715. doi: 10.1016/j.expneurol.2024.114715. Epub 2024 Feb 5.

Authors

Huaitong Yao¹, Yueru Shen², Zheng Song³, Along Han⁴, Xinghan Chen⁵, Yawen Zhang⁶, Bing Hu⁷

Affiliations

¹ Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address: yhtt@mail.ustc.edu.cn.
² Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address: syr2018@mail.ustc.edu.cn.
³ Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address: szheng@mail.ustc.edu.cn.
⁴ Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address: hanal@mail.ustc.edu.cn.
⁵ Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address: clyzmlwq@mail.ustc.edu.cn.
⁶ Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China. Electronic address: zyw1204@mail.ustc.edu.cn.
⁷ Hefei National Research Center for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China; Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China. Electronic address: bhu@ustc.edu.cn.

PMID: 38325655
DOI: 10.1016/j.expneurol.2024.114715

Abstract

Effective axon regeneration within the central nervous system (CNS) is pivotal for achieving functional recovery following spinal cord injury (SCI). Numerous extrinsic and intrinsic factors exert influences on the axon regeneration. While prior studies have demonstrated crucial involvement of specific members the Rab protein family in axon regeneration in the peripheral nervous system (PNS), the precise function of Rab11 in CNS axon regeneration in vivo remains elusive. Thus, our study aimed to elucidate the impact of Rab11 on the axon regeneration of Mauthner cells (M-cells) in zebrafish larvae. Our findings demonstrated that overexpression of Rab11bb via single-cell electroporation significantly promoted axon regeneration in individual M-cells. Conversely, knockdown of Rab11bb inhibited the axon regeneration of M-cells. RNA-seq analysis revealed an upregulation of ntng2b following Rab11bb overexpression. As we hypothesized, overexpression of Ntng2b markedly enhanced axon regeneration, while Ntng2b knockdown in the context of Rab11bb pro-regeneration substantially hindered axon regrowth. In conclusion, our study demonstrated that Rab11 promotes axon regeneration of single M-cell in the CNS through the Rab11/axon guidance/Ntng2b pathway.

Keywords: Axon guidance; CNS axon regeneration; Ntng2b; Rab11bb; Single M-cell; Zebrafish.

MeSH terms

Animals
Axon Guidance
Axons* / physiology
Nerve Regeneration
Spinal Cord Injuries*
Zebrafish