Enhanced removal of tetrabromobisphenol A by Burkholderia cepacian Y17 immobilized on biochar

Yunjiang Yu; Haobo Guo; Zijuan Zhong; Zhiyong Lu; Xiaohui Zhu; Zhenchi Li; Zhaofeng Chang

doi:10.1016/j.ecoenv.2022.114450

Enhanced removal of tetrabromobisphenol A by Burkholderia cepacian Y17 immobilized on biochar

Ecotoxicol Environ Saf. 2023 Jan 1:249:114450. doi: 10.1016/j.ecoenv.2022.114450. Epub 2022 Dec 19.

Authors

Yunjiang Yu¹, Haobo Guo¹, Zijuan Zhong², Zhiyong Lu², Xiaohui Zhu³, Zhenchi Li³, Zhaofeng Chang⁴

Affiliations

¹ Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environment Sciences, Ministry of Ecology and Environment, Guangdong 510655, China; Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in the Three Gorges Reservoir Area, Chongqing Three Gorges University, Chongqing 404000, China.
² Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environment Sciences, Ministry of Ecology and Environment, Guangdong 510655, China; Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.
³ Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environment Sciences, Ministry of Ecology and Environment, Guangdong 510655, China.
⁴ Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environment Sciences, Ministry of Ecology and Environment, Guangdong 510655, China. Electronic address: changzhaofeng545@163.com.

PMID: 38321669
DOI: 10.1016/j.ecoenv.2022.114450

Abstract

Biochar-immobilized bacteria have been widely used to remove organic pollutants; however, the enhanced effect of biochar-immobilized bacteria on tetrabromobisphenol A (TBBPA) removal has not been fully investigated and the removal mechanism remains unclear. In this study, a bacterial strain with high TBBPA degradation ability, Burkholderia cepacian Y17, was isolated from an e-waste disassembly area, immobilized with biochar, and used for the removal of TBBPA. Comparisons were performed of the factors affecting the immobilization and TBBPA removal efficiency, including the biochar preparation temperature, immobilization temperature, and pH. The highest 7-day TBBPA removal efficiency by immobilized bacteria was observed with the most suitable biochar preparation temperature (BC500) and an immobilization pH and temperature of 7 and 35 °C, respectively. The TBBPA removal efficiency reached 59.37%, which was increased by 30.23% and 15.88% compared to that of free and inactivated immobilized Y17, respectively. The suitable biochar preparation temperature BC500, immobilization temperature of 35 °C, and neutral pH of 7 increased the bacterial population and extracellular polymer concentration, which facilitated bacterial immobilization on biochar and promoted TBBPA removal. In this case, the high immobilized bacteria concentration (4.62 × 10⁸ cfu∙g^-1) and protein and polysaccharide contents (28.43 and 16.16 mg·g^-1) contributed to the removal of TBBPA by facilitating TBBPA degradation. The main TBBPA degradation processes by BC500-immobilized Y17 involved debromination, β-scission, demethylation, O-methylation, hydroxylation, and hydroxyl oxidation. This study proposes a method for preparing immobilized bacteria for TBBPA removal and enriches the microbial degradation technology for TBBPA.

Keywords: Bacteria concentration; Biochar; Burkholderia cepacian Y17; Extracellular polymeric substances; Tetrabromobisphenol A.

MeSH terms

Bacteria* / metabolism
Charcoal*
Polybrominated Biphenyls* / metabolism
Polysaccharides, Bacterial*

Substances

tetrabromobisphenol A
biochar
cepacian
Polybrominated Biphenyls
Charcoal
Polysaccharides, Bacterial