circ-Amotl1 in extracellular vesicles derived from ADSCs improves wound healing by upregulating SPARC translation

Dazhou Wu; Shengyi Chen; Dongdong Huang; Zhipeng Huang; Na Zhen; Zhenxu Zhou; Jicai Chen

doi:10.1016/j.reth.2024.01.005

circ-Amotl1 in extracellular vesicles derived from ADSCs improves wound healing by upregulating SPARC translation

Regen Ther. 2024 Jan 25:25:290-301. doi: 10.1016/j.reth.2024.01.005. eCollection 2024 Mar.

Authors

Dazhou Wu¹, Shengyi Chen¹, Dongdong Huang¹, Zhipeng Huang¹, Na Zhen¹, Zhenxu Zhou¹, Jicai Chen¹

Affiliation

¹ Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Wenzhou Medical University, PR China.

Abstract

Aim: This study aims to explore the mechanism of circ- AMOT-like protein 1 (Amotl1) in extracellular vesicles (Evs) derived from adipose-derived stromal cells (ADSCs) regulating SPARC translation in wound healing process.

Methods: The morphology, wound healing rate of the wounds and Ki67 positive rate in mouse wound healing models were assessed by H&E staining and immunohistochemistry (IHC). The binding of IGF2BP2 and SPARC was verified by RNA pull-down. Adipose-derived stromal cells (ADSCs) were isolated and verified. The Evs from ADSCs (ADSC-Evs) were analyzed.

Results: Overexpression of SPARC can promote the wound healing process in mouse models. IGF2BP2 can elevate SPARC expression to promote the proliferation and migration of HSFs. circ-Amotl1 in ADSC-Evs can increase SPARC expression by binding IGF2BP2 to promote the proliferation and migration of HSFs.

Conclusion: ADSC-Evs derived circ-Amotl1 can bind IGF2BP2 to increase SPARC expression and further promote wound healing process.

Keywords: ADSC-Evs; IGF2BP2; SPARC; Wound healing; circ-Amotl1.