Development and validation of spectrophotometric method and paper-based microfluidic devices for the quantitative determination of Amoxicillin in pure form and pharmaceutical formulations

Jwan Oday; Hind Hadi; Parween Hashim; Samantha Richardson; Alexander Iles; Nicole Pamme

doi:10.1016/j.heliyon.2024.e24968

Development and validation of spectrophotometric method and paper-based microfluidic devices for the quantitative determination of Amoxicillin in pure form and pharmaceutical formulations

Heliyon. 2024 Jan 22;10(3):e24968. doi: 10.1016/j.heliyon.2024.e24968. eCollection 2024 Feb 15.

Authors

Jwan Oday¹, Hind Hadi², Parween Hashim³, Samantha Richardson⁴, Alexander Iles⁵, Nicole Pamme⁵

Affiliations

¹ Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq.
² Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq.
³ Department of Chemistry, College of Science, University of Duhok, Duhok, Iraq.
⁴ School of Natural Sciences, University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
⁵ Department of Materials and Environmental Chemistry, Stockholm University, 106 91, Stockholm, Sweden.

Abstract

There is a growing need for easy-to-use, low cost and portable quantitative assays to determine active pharmaceutical ingredients in the pharmaceutical industry. Here, we developed a batch spectrophotometric method and a method employing a paper-based microfluidic device for the estimation of Amoxicillin (AMX) in pure solution and pharmaceutical preparations. The detection depends on the coupling reaction of Amoxicillin with diazotized sulfadimidine (DSDM) in an alkaline medium. The yellow azo dye reaction product was measured at λ_max 425 nm and linearity was observed from 2 to 30 mg L^-1 with a detection limit of 0.32 mg L^-1 and a quantification limit of 1.2 mg L^-1 was found. The reaction was then transferred onto the paper-based microfluidic device and a plateau change in color intensity was found above 10 mg L^-1. Thus, the paper-based microfluidic device can be applied for the semi-quantitative determination of Amoxicillin in pure solution and commercial pharmaceutical products for rapid screening.

Keywords: Amoxicillin (AMX); Diazotized sulfadimidine (DSDM); Paper-based microfluidic device; Pharmaceutical dosage forms; Spectrophotometry.