In this work, a novel covalent organic frame (TAPT-TFPB COF) with self-enhanced photoelectric activity was prepared for decorating on conductive single-walled carbon nanotubes (SWCNT) to synthetize a high-performance photoelectric nanocomposite (COF/SWCNT), in which the interfacial charge separation and photogenerated carrier migration rate was significantly improved to obtain desiring photoelectric conversion efficiency for generating an extremely high photocurrent. Accordingly, the synthetic COF/SWCNT was ingeniously applied in the fabrication of ultrasensitive photoelectrochemical (PEC) biosensor for realizing the trace ATP detection by integrating with an Exo III-assisted dual DNA recycling amplification strategy. The recycling amplification could efficiently convert trace target ATP into plentiful output DNA, which ingeniously triggered the hybridization chain reaction (HCR) to generate a long DNA strand with substantial quencher manganese porphyrin (MnPP) loading to depress the photocurrent of COF/SWCNT. The experimental data showed that proposed biosensor had a detection range from 10 fmol L-1 to 10 nmol L-1 with the detection limit as low as 2.75 fmol L-1 (S/N = 3). In addition, this proposed biosensor showed excellent analytical performance in terms of stability, specificity and reproducibility, providing a possibility to accomplish sensitive and accurate in vitro diagnosis.
Keywords: Photochemical biosensor; Photoelectric conversion efficiency; Self-enhancement; Sensitive and accurate detection.
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