Targeted Analysis of Glycerophospholipids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer

Hui Chen; Sylvère Durand; Mélanie Bourgin; Flavia Lambertucci; Omar Motiño; Léa Montégut; Sijing Li; Uxía Nogueira-Recalde; Gerasimos Anagnostopoulos; Maria Chiara Maiuri; Guido Kroemer; Isabelle Martins

doi:10.1007/978-1-0716-3694-7_14

Targeted Analysis of Glycerophospholipids and Mono-, Di-, or Tri-Acylglycerides in Liver Cancer

Methods Mol Biol. 2024:2769:189-198. doi: 10.1007/978-1-0716-3694-7_14.

Authors

Hui Chen^{1

2

3}, Sylvère Durand^{1

2}, Mélanie Bourgin^{1

2}, Flavia Lambertucci^{1

2}, Omar Motiño^{1

2}, Léa Montégut^{1

2

3}, Sijing Li^{1

2

3}, Uxía Nogueira-Recalde^{1

2

4}, Gerasimos Anagnostopoulos^{1

2}, Maria Chiara Maiuri^{1

2

5}, Guido Kroemer^{1

2

6}, Isabelle Martins^{7

8}

Affiliations

¹ Centre de Recherche des Cordeliers, Equipe labellisée par la Ligue contre le cancer, Inserm U1138, Université Paris Cité, Sorbonne Université, Institut Universitaire de France, Paris, France.
² Metabolomics and Cell Biology Platforms, UMS AMMICa, Gustave Roussy, Villejuif, France.
³ Faculté de Médecine, Université de Paris Saclay, Kremlin Bicêtre, France.
⁴ Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Fundación Profesor Novoa Santos, A Coruña, Spain.
⁵ Department of Molecular Medicine and Medical Biotechnologies, University of Napoli Federico II, Naples, Italy.
⁶ Institut du Cancer Paris CARPEM, Department of Biology, Hôpital Européen Georges Pompidou, AP-HP, Paris, France.
⁷ Centre de Recherche des Cordeliers, Equipe labellisée par la Ligue contre le cancer, Inserm U1138, Université Paris Cité, Sorbonne Université, Institut Universitaire de France, Paris, France. isabelle.martins@inserm.fr.
⁸ Metabolomics and Cell Biology Platforms, UMS AMMICa, Gustave Roussy, Villejuif, France. isabelle.martins@inserm.fr.

PMID: 38315398
DOI: 10.1007/978-1-0716-3694-7_14

Abstract

The metabolic rearrangements of hepatic metabolism associated with liver cancer are still incompletely understood. There is an ongoing need to identify novel and more efficient diagnostic biomarkers and therapeutic targets based on the metabolic mechanisms of these diseases. In comparison to traditional diagnostic biomarkers, metabolomics is a comprehensive technique for discovering chemical signatures for liver cancer screening, prediction, and earlier diagnosis. Lipids are a large and diverse group of complex biomolecules that are at the heart of liver physiology and play an important role in the development and progression of cancer. In this chapter, we described two detailed protocols for targeted lipids analysis: glycerophospholipids and mono, di, tri-acylglycerides, both by Flow Injection Analysis (FIA) HPLC coupled to a SelexIon/QTRAP 6500+ system. These approaches provide a targeted lipidomic metabolomic signature of dissimilar metabolic disorders affecting liver cancers.

Keywords: Bi-acylglycerides; Glycerophospholipids; Lipids; Liver cancer; Mono-acylglycerides; SelexIon/QTRAP 6500+ system; Targeted analysis; Tri-acylglycerides.

MeSH terms

Biomarkers
Glycerophospholipids*
Humans
Liver Neoplasms*
Metabolomics / methods

Substances

Glycerophospholipids
Biomarkers