Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches

Cristian D Gutierrez Reyes; Akeem Sanni; Damir Mogut; Moyinoluwa Adeniyi; Parisa Ahmadi; Mojgan Atashi; Sherifdeen Onigbinde; Yehia Mechref

doi:10.1007/978-1-0716-3666-4_15

Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches

Methods Mol Biol. 2024:2762:251-266. doi: 10.1007/978-1-0716-3666-4_15.

Authors

Cristian D Gutierrez Reyes^#¹, Akeem Sanni^#¹, Damir Mogut², Moyinoluwa Adeniyi¹, Parisa Ahmadi¹, Mojgan Atashi¹, Sherifdeen Onigbinde¹, Yehia Mechref³

Affiliations

¹ Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
² Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
³ Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA. Yehia.Mechref@ttu.edu.

^# Contributed equally.

PMID: 38315370
DOI: 10.1007/978-1-0716-3666-4_15

Abstract

Targeted mass spectrometric analysis is widely employed across various omics fields as a validation strategy due to its high sensitivity and accuracy. The approach has been successfully employed for the structural analysis of proteins, glycans, lipids, and metabolites. Multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) have been the methods of choice for targeted structural studies of biomolecules. These target analyses simplify the analytical workflow, reduce background interference, and increase selectivity/specificity, allowing for a reliable quantification of permethylated N-glycans in complex biological matrices.

Keywords: MRM; PRM; Permethylated N-glycans.

MeSH terms

Mass Spectrometry / methods
Polysaccharides* / chemistry
Workflow

Substances

Polysaccharides