Bi-allelic variants in DNAH3 cause male infertility with asthenoteratozoospermia in humans and mice

Gui-Quan Meng; Yaling Wang; Chen Luo; Yu-Mei Tan; Yong Li; Chen Tan; Chaofeng Tu; Qian-Jun Zhang; Liang Hu; Huan Zhang; Lan-Lan Meng; Chun-Yu Liu; Leiyu Deng; Guang-Xiu Lu; Ge Lin; Juan Du; Yue-Qiu Tan; Yanwei Sha; Lingbo Wang; Wen-Bin He

doi:10.1093/hropen/hoae003

Bi-allelic variants in DNAH3 cause male infertility with asthenoteratozoospermia in humans and mice

Hum Reprod Open. 2024 Jan 11;2024(1):hoae003. doi: 10.1093/hropen/hoae003. eCollection 2024.

Authors

Gui-Quan Meng^{1

2

3}, Yaling Wang⁴, Chen Luo^{2

3}, Yu-Mei Tan⁵, Yong Li^{2

3}, Chen Tan^{2

3}, Chaofeng Tu^{2

3}, Qian-Jun Zhang^{1

2

3}, Liang Hu^{2

3}, Huan Zhang^{2

3}, Lan-Lan Meng^{2

3}, Chun-Yu Liu⁶, Leiyu Deng⁷, Guang-Xiu Lu^{1

2

3}, Ge Lin^{1

2

3}, Juan Du^{1

2

3}, Yue-Qiu Tan^{1

2

3}, Yanwei Sha^{8

9

10}, Lingbo Wang⁴, Wen-Bin He^{1

2

3}

Affiliations

¹ Genetic Department, Hunan Guangxiu Hospital, Hunan Normal University School of Medicine, Changsha, China.
² National Engineering and Research Center of Human Stem Cells & Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China.
³ Genetic Department, Reproductive and Genetic Hospital of CITIC-Xiangya & Clinical Research Center for Reproduction and Genetics in Hunan Province, Changsha, Hunan, China.
⁴ Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Institute of Reproduction and Development, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China.
⁵ GuangDong Provincial Fertility Hospital (GuangDong Provincial Reproductive Science Institute), Guangzhou, China.
⁶ Obstetrics and Gynecology Hospital, State Key Laboratory of Genetic Engineering, Institute of Reproduction and Development, Fudan University, Shanghai, China.
⁷ Reproductive Center of No.924 Hospital of PLA Joint Logistic Support Force, Guilin, China.
⁸ Department of Andrology, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, China.
⁹ Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, China.
¹⁰ State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.

Abstract

Study question: Are there other pathogenic genes for asthenoteratozoospermia (AT)?

Summary answer: DNAH3 is a novel candidate gene for AT in humans and mice.

What is known already: AT is a major cause of male infertility. Several genes underlying AT have been reported; however, the genetic aetiology remains unknown in a majority of affected men.

Study design size duration: A total of 432 patients with AT were recruited in this study. DNAH3 mutations were identified by whole-exome sequencing (WES). Dnah3 knockout mice were generated using the genome editing tool. The morphology and motility of sperm from Dnah3 knockout mice were investigated. The entire study was conducted over 3 years.

Participants/materials setting methods: WES was performed on 432 infertile patients with AT. In addition, two lines of Dnah3 knockout mice were generated. Haematoxylin and eosin (H&E) staining, transmission electron microscopy (TEM), immunostaining, and computer-aided sperm analysis (CASA) were performed to investigate the morphology and motility of the spermatozoa. ICSI was used to overcome the infertility of one patient and of the Dnah3 knockout mice.

Main results and the role of chance: DNAH3 biallelic variants were identified in three patients from three unrelated families. H&E staining revealed various morphological abnormalities in the flagella of sperm from the patients, and TEM and immunostaining further showed the loss of the central pair of microtubules, a dislocated mitochondrial sheath and fibrous sheath, as well as a partial absence of the inner dynein arms. In addition, the two Dnah3 knockout mouse lines demonstrated AT. One patient and the Dnah3 knockout mice showed good treatment outcomes after ICSI.

Large scale data: N/A.

Limitations reasons for caution: This is a preliminary report suggesting that defects in DNAH3 can lead to asthenoteratozoospermia in humans and mice. The pathogenic mechanism needs to be further examined in a future study.

Wider implications of the findings: Our findings show that DNAH3 is a novel candidate gene for AT in humans and mice and provide crucial insights into the biological underpinnings of this disorder. The findings may also be beneficial for counselling affected individuals.

Study funding/competing interests: This work was supported by grants from National Natural Science Foundation of China (82201773, 82101961, 82171608, 32322017, 82071697, and 81971447), National Key Research and Development Program of China (2022YFC2702604), Scientific Research Foundation of the Health Committee of Hunan Province (B202301039323, B202301039518), Hunan Provincial Natural Science Foundation (2023JJ30716), the Medical Innovation Project of Fujian Province (2020-CXB-051), the Science and Technology Project of Fujian Province (2023D017), China Postdoctoral Science Foundation (2022M711119), and Guilin technology project for people's benefit (20180106-4-7). The authors declare no competing interests.

Keywords: DNAH3; asthenoteratozoospermia; bi-allelic variants; male infertility; sperm flagella.