Physiological and transcriptomic analysis revealed that the accumulation of reactive oxygen species caused the low temperature sensitivity of Liriodendron × sinoamericanum

Ye Lu; Lingfeng Hu; Long Yu; Shuang Liang; Haoxian Qu; Mingqi Wang; Zhaodong Hao; Liming Yang; Jisen Shi; Jinhui Chen

doi:10.1016/j.plantsci.2024.112020

Physiological and transcriptomic analysis revealed that the accumulation of reactive oxygen species caused the low temperature sensitivity of Liriodendron × sinoamericanum

Plant Sci. 2024 May:342:112020. doi: 10.1016/j.plantsci.2024.112020. Epub 2024 Feb 3.

Authors

Ye Lu¹, Lingfeng Hu¹, Long Yu¹, Shuang Liang¹, Haoxian Qu¹, Mingqi Wang¹, Zhaodong Hao¹, Liming Yang², Jisen Shi³, Jinhui Chen⁴

Affiliations

¹ State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in the Southern China, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
² State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in the Southern China, Nanjing Forestry University, Nanjing 210037, China.
³ State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in the Southern China, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China. Electronic address: jshi@njfu.edu.cn.
⁴ State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in the Southern China, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China. Electronic address: chenjh@njfu.edu.cn.

PMID: 38311251
DOI: 10.1016/j.plantsci.2024.112020

Abstract

Liriodendron × sinoamericanum is widely cultivated in southern China as an excellent wood and garden ornamental trees. However, its intolerance to low temperature limits its application to high latitudes. Understanding the molecular mechanism of low temperature sensitivity of Liriodendron × sinoamericanum is very important for its further application. In this study, combined with physiological and transcriptomic analysis, it was revealed that low temperature stress can lead to water loss and decreased photosynthetic capacity of Liriodendron × sinoamericanum leaves. The accelerated accumulation of reactive oxygen species (ROS) caused by the imbalance of cell REDOX homeostasis is one of the important reasons for the low temperature sensitivity. Further analysis showed that several transcription factors could be involved in regulating the synthesis and degradation of ROS, among which LsNAC72 and LsNAC73a could regulate the accumulation of O₂^- and H₂O₂ in leaves by affecting the expression level of LsAPX, LsSOD, LsPAO, and LsPOD.

Keywords: Cold stress; Liriodendron × sinoamericanum; Reactive oxygen species; Transcriptome.

MeSH terms

Gene Expression Profiling
Hydrogen Peroxide
Liriodendron* / genetics
Reactive Oxygen Species / metabolism
Temperature

Substances

Reactive Oxygen Species
Hydrogen Peroxide