Geometric transformation adaptive optics (GTAO) for volumetric deep brain imaging through gradient-index lenses

Yuting Li; Zongyue Cheng; Chenmao Wang; Jianian Lin; Hehai Jiang; Meng Cui

doi:10.1038/s41467-024-45434-5

Geometric transformation adaptive optics (GTAO) for volumetric deep brain imaging through gradient-index lenses

Nat Commun. 2024 Feb 3;15(1):1031. doi: 10.1038/s41467-024-45434-5.

Authors

Yuting Li^#^{1

2}, Zongyue Cheng^#^{1

2}, Chenmao Wang^{1

2}, Jianian Lin^{1

2}, Hehai Jiang^{1

2}, Meng Cui^{3

4

5}

Affiliations

¹ School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA.
² Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA.
³ School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN, 47907, USA. mengcui@purdue.edu.
⁴ Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47907, USA. mengcui@purdue.edu.
⁵ Department of Biology, Purdue University, West Lafayette, IN, 47907, USA. mengcui@purdue.edu.

^# Contributed equally.

Abstract

The advance of genetic function indicators has enabled the observation of neuronal activities at single-cell resolutions. A major challenge for the applications on mammalian brains is the limited optical access depth. Currently, the method of choice to access deep brain structures is to insert miniature optical components. Among these validated miniature optics, the gradient-index (GRIN) lens has been widely employed for its compactness and simplicity. However, due to strong fourth-order astigmatism, GRIN lenses suffer from a small imaging field of view, which severely limits the measurement throughput and success rate. To overcome these challenges, we developed geometric transformation adaptive optics (GTAO), which enables adaptable achromatic large-volume correction through GRIN lenses. We demonstrate its major advances through in vivo structural and functional imaging of mouse brains. The results suggest that GTAO can serve as a versatile solution to enable large-volume recording of deep brain structures and activities through GRIN lenses.

MeSH terms

Animals
Brain / diagnostic imaging
Lens, Crystalline*
Lenses*
Mammals
Mice
Neuroimaging
Optics and Photonics

Abstract

MeSH terms

Grants and funding