Non-proteolytic ubiquitination of HBx controls HBV replication

Xiangpeng Sheng; Yi Yang; Min Zhu; Linlin Zhou; Fang Zhu; Yuanfei Zhu; Siying Dong; Hui Kong; Honghua Wang; Ji Jiang; Mingyue Wan; Mingyang Feng; Qiang Deng; Yumin Xu; Qing You; Ronggui Hu

doi:10.1016/j.virs.2024.01.008

Non-proteolytic ubiquitination of HBx controls HBV replication

Virol Sin. 2024 Apr;39(2):338-342. doi: 10.1016/j.virs.2024.01.008. Epub 2024 Feb 1.

Authors

Xiangpeng Sheng¹, Yi Yang², Min Zhu³, Linlin Zhou⁴, Fang Zhu⁵, Yuanfei Zhu⁶, Siying Dong⁷, Hui Kong⁸, Honghua Wang⁹, Ji Jiang³, Mingyue Wan¹⁰, Mingyang Feng¹⁰, Qiang Deng⁶, Yumin Xu¹¹, Qing You¹², Ronggui Hu¹³

Affiliations

¹ State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China; State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. Electronic address: shengxiangpeng@ucas.ac.cn.
² Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
³ Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
⁴ Department of Pathogenic Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
⁵ School of Medicine, Guizhou University, Guiyang 550025, China.
⁶ Key Laboratory of Medical Molecular Virology (MOE & MOH), School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
⁷ State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China.
⁸ State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
⁹ Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
¹⁰ Department of Hospital Infection Management, Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
¹¹ Department of Hospital Infection Management, Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: xym121@163.com.
¹² School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: youqing@sjtu.edu.cn.
¹³ State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. Electronic address: coryhu@sibcb.ac.cn.

Abstract

•
The expression level of TRIM21 in patients is negatively correlated with the replication and integration of HBV.
•
TRIM21 was found to trigger non-proteolytic ubiquitination of X protein of HBV.
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This study proposes that the PRYSPRY and RING domains in TRIM21 dimer can form a docking conformation for HBx binding.
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TRIM21-mediated HBx ubiquitination disrupts the DDB1 recruitment to HBx and stabilize Smc6.

Publication types

Letter

MeSH terms

Hepatitis B virus* / genetics
Hepatitis B virus* / physiology
Host-Pathogen Interactions
Humans
Trans-Activators* / genetics
Trans-Activators* / metabolism
Ubiquitination*
Viral Regulatory and Accessory Proteins* / genetics
Viral Regulatory and Accessory Proteins* / metabolism
Virus Replication*

Substances

Viral Regulatory and Accessory Proteins
hepatitis B virus X protein
Trans-Activators