Animal models are extensively used in all aspects of biomedical research, with substantial contributions to our understanding of diseases, the development of pharmaceuticals, and the exploration of gene functions. The field of genome modification in rabbits has progressed slowly. However, recent advancements, particularly in CRISPR/Cas9-related technologies, have catalyzed the successful development of various genome-edited rabbit models to mimic diverse diseases, including cardiovascular disorders, immunodeficiencies, aging-related ailments, neurological diseases, and ophthalmic pathologies. These models hold great promise in advancing biomedical research due to their closer physiological and biochemical resemblance to humans compared to mice. This review aims to summarize the novel gene-editing approaches currently available for rabbits and present the applications and prospects of such models in biomedicine, underscoring their impact and future potential in translational medicine.
动物模型被广泛应用于生物医学研究的各个领域，为疾病调查、药物开发和基因功能研究做出了重要贡献。多年来，兔子的基因组改造进展缓慢。然而，近年来CRISPR/Cas9 相关技术的进步，促进了大量基因组编辑兔模型的成功开发。这些模型兔可有效模拟多种疾病，包括心血管疾病、免疫缺陷、衰老相关疾病、神经系统疾病、眼科病变等。与小鼠相比，基因组编辑兔模型在生理和生化方面更接近人类，因此在推动生物医学研究方面大有可为。在这篇综述中，我们总结了可用于基因组编辑兔的新型基因编辑方法，并介绍了基因组编辑家兔模型在生物医学中的应用和前景，旨在强调它们在转化医学中的特殊影响和未来潜力。.
Keywords: Animal model; CRISPR/Cas9; Genetic diseases; Genome editing; Rabbit.