IFT80 promotes early bone healing of tooth sockets through the activation of TAZ/RUNX2 pathway

Ziwei Zhao; Ying Geng; Qiaoqi Ni; Yue Chen; Yanan Cao; Yahui Lu; Hua Wang; Ruixia Wang; Wen Sun

doi:10.1111/odi.14873

IFT80 promotes early bone healing of tooth sockets through the activation of TAZ/RUNX2 pathway

Oral Dis. 2024 Jan 29. doi: 10.1111/odi.14873. Online ahead of print.

Authors

Ziwei Zhao^{1

2

3}, Ying Geng^{1

2}, Qiaoqi Ni^{1

2}, Yue Chen^{1

2}, Yanan Cao^{2

3}, Yahui Lu^{1

2}, Hua Wang^{1

2

4}, Ruixia Wang^{2

3

4}, Wen Sun^{1

2

4}

Affiliations

¹ Department of Basic Science of Stomatology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
² Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
³ Department of Dental Implantology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
⁴ Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China.

PMID: 38287672
DOI: 10.1111/odi.14873

Abstract

Intraflagellar transport (IFT) proteins have been reported to regulate cell growth and differentiation as the essential functional component of primary cilia. The effects of IFT80 on early bone healing of extraction sockets have not been well studied. To investigate whether deletion of Ift80 in alveolar bone-derived mesenchymal stem cells (aBMSCs) affected socket bone healing, we generated a mouse model of specific knockout of Ift80 in Prx1 mesenchymal lineage cells (Prx1^Cre ;IFT80^f/f ). Our results demonstrated that deletion of IFT80 in Prx1 lineage cells decreased the trabecular bone volume, ALP-positive osteoblastic activity, TRAP-positive osteoclastic activity, and OSX-/COL I-/OCN-positive areas in tooth extraction sockets of Prx1^Cre ; IFT80^f/f mice compared with IFT80^f/f littermates. Furthermore, aBMSCs from Prx1^Cre ; IFT80^f/f mice showed significantly decreased osteogenic markers and downregulated migration and proliferation capacity. Importantly, the overexpression of TAZ recovered significantly the expressions of osteogenic markers and migration capacity of aBMSCs. Lastly, the local administration of lentivirus for TAZ enhanced the expression of RUNX2 and OSX and promoted early bone healing of extraction sockets from Prx1^Cre ; IFT80^f/f mice. Thus, IFT80 promotes osteogenesis and early bone healing of tooth sockets through the activation of TAZ/RUNX2 pathway.

Keywords: IFT80; RUNX2; TAZ; alveolar bone-derived mesenchymal stem cells; bone healing; primary cilia; tooth socket extraction.

Abstract

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