Exploring the efficacy of a novel prebiotic-like growth promoter on broiler chicken production performance

Zulfqarul Haq; Ankur Rastogi; Ramesh Kumar Sharma; Pratiksha Raghuwanshi; Maninder Singh; Azmat Alam Khan; Syed Mudasir Ahmad

doi:10.1016/j.vas.2023.100331

Exploring the efficacy of a novel prebiotic-like growth promoter on broiler chicken production performance

Vet Anim Sci. 2023 Dec 30:23:100331. doi: 10.1016/j.vas.2023.100331. eCollection 2024 Mar.

Authors

Zulfqarul Haq¹, Ankur Rastogi², Ramesh Kumar Sharma², Pratiksha Raghuwanshi³, Maninder Singh⁴, Azmat Alam Khan⁵, Syed Mudasir Ahmad⁶

Affiliations

¹ Indian Council of Medical Research Project, Division of L.P.M, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India.
² Division of Animal Nutrition, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India.
³ Division of Veterinary Biochemistry and Physiology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India.
⁴ Department of Veterinary Public Health and Epidemiology, Guru Angad Dev Veterinary and Animal Sciences University, Rampura Phul, India.
⁵ Division of LPM, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India.
⁶ Division of Animal Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India.

Abstract

This study attempts to isolate a candidate growth promoter from the ovine paunch waste and scrutinize its effects on the production performance of broiler chickens as compared to mannan-oligosaccharide (MOS), a prebiotic, and lincomycin, an antibiotic growth promoter (AB). The paunch waste collected from slaughtered sheep was processed to remove particulate matter. The clarified liquid was then added to an excess of ethanol (1:9 ratio), and the resultant precipitate {(novel growth-promoting paunch extract (NGPE)} was collected, dried, and stored. In vitro increase in cell density for probiotic bacteria viz. Lactobacillus rhamnosus and Enterococcus faecalis (Log₁₀ CFU/ml) were significantly higher (P < 0.01) in NGPE supplemented media (2.78 ± 0.11 and 2.77 ± 0.10) as compared to that on MOS (1.28 ± 0.05 and 2.49 ± 0.09) and glucose (1.09 ± 0.04 and 1.12 ± 0.04) supplemented media. In the in-vivo trial of six weeks duration with broiler chickens (Cobb-400), NGPE supplementation resulted in significantly higher growth in weeks IV (P < 0.05) and VI (P < 0.01) of age in comparison to MOS and AGP supplemented groups, a lower (P < 0.01) cumulative feed conversion ratio in comparison to MOS supplemented groups, and a higher (P < 0.01) cumulative protein efficiency ratio compared to MOS and AGP supplementation. NGPE supplementation also lowered lipid peroxidation (P < 0.01), increased reduced glutathione activity (P < 0.01) in chicken erythrocytes, and boosted the lactic acid bacteria count in the cecal contents (P < 0.01). This is the first report of the isolation of a paunch waste extract that increased the in vitro growth of probiotic bacteria and improved the production performance of broiler chickens.

Keywords: Antibiotics; Antimicrobial resistance; Chicken; Novel growth promoter; Prebiotic.