The complete chloroplast genome sequence of Malus × adstringens Zabel 'Hopa' (Rosaceae)

Leiming Dong; Ruizhen Wang; Hengxing Liu; Guowei Xia; Jian Quan; Ling Guo; Minghui Chen

doi:10.1080/23802359.2023.2292158

The complete chloroplast genome sequence of Malus × adstringens Zabel 'Hopa' (Rosaceae)

Mitochondrial DNA B Resour. 2024 Jan 25;9(1):173-177. doi: 10.1080/23802359.2023.2292158. eCollection 2024.

Authors

Leiming Dong¹, Ruizhen Wang¹, Hengxing Liu¹, Guowei Xia², Jian Quan¹, Ling Guo¹, Minghui Chen¹

Affiliations

¹ Key Laboratory of National Forestry and Grassland Administration on Plant Ex situ Conservation, Beijing Floriculture Engineering Technology Research Centre, Beijing Botanical Garden, Beijing, China.
² College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, China.

Abstract

Malus × adstringens Zabel 'Hopa' is an important crabapple cultivar with significant ornamental value. Here, we assembled its complete chloroplast (cp) genome using the next-generation sequencing technology to clarify the phylogenetic relationships in Malus. The total length of the complete chloroplast genome was 160,230 base pairs (bp) with a GC content of 36.50%, consisting of a large single-copy (LSC) region with a sequence length of 88,310 bp, a small single-copy (SSC) region with a sequence length of 19,196 bp, and a pair of inverted repeat (IR) regions of 26,362 bp. The complete chloroplast genome contained 128 genes, namely 84 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. In addition, 73 SSRs were found in the M. 'Hopa' cp genome. The phylogenetic relationship of M. 'Hopa' in Malus is closely related to M. spectabilis (Aiton) Borkh. and then to M. sieversii (Lebed.) M. Roem. Our results demonstrate that it is feasible to resolve the phylogenetic relationships of crabapple cultivars and identify their putative maternal lineages using cp genomic data.

Keywords: Malus; chloroplast genome; crabapple; phylogeny relationships.

Grants and funding

This work was supported by the Beijing Natural Science Foundation (6224061) and Beijing Municipal Administration Center of Parks (kjxx2021004, zx2022010, zx2022013).