Salmonella enterica serovar Meleagridis (S. Meleagridis) is a non-typhoidal Salmonella serotype commonly found in food and humans. In this study, we investigated 61 Chinese S. Meleagridis isolates from various sources, predominantly from pigs and pig products. Additionally, the serotype was also identified in samples from human infections. Whole-genome sequencing analysis of these isolates, combined with 10 isolates from other countries, demonstrated that the Chinese isolates formed a distinct Cluster C, further divided into two subclusters (Cluster C-1 and Cluster C-2) based on cgMLST analysis. CRISPR typing divided the 61 isolates into three CRISPR types (MCT1, MCT2, MCT3), belonging to Cluster I (96.7%, 59/61) and Cluster II (3.3%, 2/61), which corresponded to Cluster C-2 and Cluster C-1, respectively. Among the 48 identified spacers, the spacer SoeB5 was the only target differentiating MCT1 and MCT2 isolates of Cluster I. MelB12 and MelB13, identified in US and Denmark isolates, were not found among the 61 Chinese isolates. Examination of antimicrobial resistance gene profiles and their genetic contexts uncovered the presence of IncR plasmids in 43 (70.5%, 43/61) isolates within Cluster C, conferring resistance to tetracycline and trimethoprim/sulfamethoxazole. Homology analysis of spacers showed that 12 spacers exhibited similarity to sequences in phages or plasmids. Additionally, five spacers showed homology to sequences in plasmids from other Salmonella serotypes, suggesting their potential role in helping S. Meleagridis resist against Salmonella isolates carrying similar plasmids. The comprehensive analysis of CRISPR, cgMLST, and antimicrobial resistance in S. Meleagridis highlights the pig reservoir as a crucial factor in the evolution and transmission of this serotype to humans.
Keywords: CRISPR; Pig; Salmonella enterica serovar Meleagridis (S. Meleagridis); cgMLST, Antimicrobial resistance.
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