Green Synthesis of Polylactic acid/Fe3O4@β-Cyclodextrin Nanofibrous Nanocomposite Loaded with Ferulago Angulata Extract as a Novel Nano-biosorbent: Evaluation of Diazinon Removal and Antibacterial Activity

Abstract

Background: Organophosphate pesticides are one of the most extensively applied insecticides in agriculture. These insecticides persist in the environs and thereby cause severe pollution problems. Iron oxide polymer nanocomposites are wastewater remediation agents synthesized by various methods. When compared to chemical processes, green synthesis using plant extract is thought to be more cost- and environmentally-friendly.

Objectives: This study aimed to evaluate the green synthesis of Fe₃O₄@β-Cyclodextrin (Fe₃O₄@β-CD) nanoparticles using Ferulago angulata (F. angulata) methanol extract. These nanoparticles are loaded on polylactic acid (PLA) nanofibrous nanocomposite along with Ferulago angulata extract (2, 4, and, 6wt %) to produce PLA/Fe₃O₄@β-CD/F. angulata extract nanofibrous nanocomposite as a new nano biosorbent. Furthermore, the antibacterial properties of this compound and its activity in diazinon removal have been evaluated.

Materials and methods: Fe₃O₄@β-CD nanoparticles synthesis was performed via co-precipitation method using FeCl₃.6H₂O and FeCl₂.4H₂O and β-cyclodextrin, and Ferulago angulata extract. Then polylactic acid/ Fe₃O₄@β-CD / F. angulate.extract nanofibrous nanocomposite was prepared by the electrospinning method. Energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction analysis (XRD), vibrating sample magnetometer (VSM), and Fourier transform infrared spectroscopy (FTIR) were used to analyze the structure of the nanocomposite. The antibacterial activity of this nanocomposite against several fish and human bacterial pathogens, as well as its effectiveness in diazinon elimination, have been evaluated in the sections that follow.

Results: The nanocomposite structure demonstrated that Fe₃O₄ nanoparticles were produced and put into the polylactic acid matrix with an average particle size of 40 nm. Furthermore, the results showed that this nanocomposite exhibited removal efficiency of diazinon over 80% after 120 minutes under pH=7 and 2.5 gr.L^-1 nanocomposite concentration. Also, this structure showed above 70% antibacterial ability against Bacillus cereus, Staphylococcus epidermidis and 60% antibacterial ability against Streptococcus iniae and Yersinia ruckeri.

Conclusion: Fe₃O₄ nanocomposite synthesis may be accomplished in a delicate and efficient manner by using Ferulago angulata to produce Fe₃O₄@-CD nanoparticles. The stability of the nanoparticles was enhanced by combining Ferulago angulata extract with polylactic acid nanofibers to create an antibacterial homocomposition nanocomposite. This device may be used to remove and disinfect diazinon from aqueous media in an environmentally friendly manner.

Keywords: Diazinon; Iron oxide nanoparticles; Nanocomposite; Polylactic acid; Ferulago Angulata.