A fast and sensitive aptasensor was developed using nanoplates with peroxidase activity as a novel approach. E. coli detection is described using a silver/platinum nanoplate (Ag/Pt NPL) that interacts with an oligonucleotide aptamer as a bioreceptor. The size of the Ag/Pt NPLs was about 42 nm according to the FE-SEM images. The EDS result indicates that a thin layer of Pt ions was coated on the surface of the Ag NPLs. This nanobiosensor has the ability to specifically bind to E. coli, increasing the peroxidase activity of the apt-Ag/Pt NPL. Finally, the blue color of the solution in the contaminated water samples was increased in the presence of 3,3',5,5'-tetramethylbenzidine (TMB) as a substrate and H2O2. The assay can be completed in 30 min and the presence of E. coli levels can be distinguished with the naked eye. The absorbance at 652 nm is proportional to pathogen concentration from 10 to 108 CFU·mL-1, with a detection limit of 10 CFU·mL-1. The percent recovery for the water samples spiked with E. coli is 95%. The developed assay should serve as a general platform for detecting other pathogenic bacteria which affect water and food quality. The proposed E. coli detection strategy has appealing characteristics such as high sensitivity, simple operation, short testing time, and low cost.
Keywords: E. coli; aptamer; biosensor; detection; peroxidase activity; silver/platinum nanoplate.
Copyright © 2024, Pebdeni et al.