Field phenotyping of ten wheat cultivars under elevated CO2 shows seasonal differences in chlorophyll fluorescence, plant height and vegetation indices

Abstract

In the context of climate change and global sustainable development goals, future wheat cultivation has to master various challenges at a time, including the rising atmospheric carbon dioxide concentration ([CO₂]). To investigate growth and photosynthesis dynamics under the effects of ambient (~434 ppm) and elevated [CO₂] (~622 ppm), a Free-Air CO₂ Enrichment (FACE) facility was combined with an automated phenotyping platform and an array of sensors. Ten modern winter wheat cultivars (Triticum aestivum L.) were monitored over a vegetation period using a Light-induced Fluorescence Transient (LIFT) sensor, ground-based RGB cameras and a UAV equipped with an RGB and multispectral camera. The LIFT sensor enabled a fast quantification of the photosynthetic performance by measuring the operating efficiency of Photosystem II (F_q'/F_m') and the kinetics of electron transport, i.e. the reoxidation rates F_r1' and F_r2'. Our results suggest that elevated [CO₂] significantly increased F_q'/F_m' and plant height during the vegetative growth phase. As the plants transitioned to the senescence phase, a pronounced decline in F_q'/F_m' was observed under elevated [CO₂]. This was also reflected in the reoxidation rates F_r1' and F_r2'. A large majority of the cultivars showed a decrease in the harvest index, suggesting a different resource allocation and indicating a potential plateau in yield progression under e[CO₂]. Our results indicate that the rise in atmospheric [CO₂] has significant effects on the cultivation of winter wheat with strong manifestation during early and late growth.

Keywords: CO2; FACE (Free-Air CO2 Enrichment); chlorophyll; climate change; fluorescence; phenotyping; senescence; wheat.