Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids Paphiopedilum SCBG Huihuang90

Beiyi Guo; Hong Chen; Yuying Yin; Wei Wang; Songjun Zeng

doi:10.3390/plants13020197

Tissue Culture via Protocorm-like Bodies in an Orchids Hybrids Paphiopedilum SCBG Huihuang90

Plants (Basel). 2024 Jan 11;13(2):197. doi: 10.3390/plants13020197.

Authors

Beiyi Guo¹, Hong Chen¹, Yuying Yin², Wei Wang¹, Songjun Zeng²

Affiliations

¹ Guangzhou Collaborative Innovation Center on Science-Tech of Ecology and Landscape, Guangzhou Landscape Plant Germplasm Resource Nursery, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China.
² Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Abstract

This study successfully established an efficient in vitro propagation system for Paphiopedilum SCBG Huihuang90 via protocorm-like body (PLB) formation from seed-derived calluses, PLB proliferation and differentiation, root induction and greenhouse acclimatization. Furthermore, 1/2 Murashige and Skoog (MS) + 0.025 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) was suitable for the proliferation of PLBs, and 1/2MS + 10% coconut water (CW, v/v) + 0.5 g/L activated carbon (AC) was suitable for PLB differentiation. PLBs at different developmental stages required different kinds of sugars. This study provided a reference for research on the propagation techniques of other Paphiopedilum.

Keywords: Paphiopedilum; in vitro propagation; protocorm-like body.

Grants and funding

AB2018002, AB2018026, KF202006/Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, and Chinese Academy of Sciences