Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?

Saida Mebarek; Rene Buchet; Slawomir Pikula; Agnieszka Strzelecka-Kiliszek; Leyre Brizuela; Giada Corti; Federica Collacchi; Genevieve Anghieri; Andrea Magrini; Pietro Ciancaglini; Jose Luis Millan; Owen Davies; Massimo Bottini

doi:10.3390/biom14010042

Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?

Biomolecules. 2023 Dec 28;14(1):42. doi: 10.3390/biom14010042.

Authors

Affiliations

¹ Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, Université de Lyon, Université Claude Bernard Lyon 1, 69 622 Villeurbanne Cedex, France.
² Laboratory of Biochemistry of Lipids, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland.
³ Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.
⁴ School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough LE113TU, UK.
⁵ Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy.
⁶ Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-901, São Paulo, Brazil.
⁷ Sanford Children's Health Research Center, Sanford Burnham Prebys, La Jolla, CA 92037, USA.

Abstract

Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extracellular matrix (matrix vesicles). Media vesicles are purified directly from the extracellular medium. On the other hand, matrix vesicles are purified after discarding the extracellular medium and subjecting the cells embedded in the extracellular matrix or bone or cartilage tissues to an enzymatic treatment. Several pieces of experimental evidence indicated that matrix vesicles and media vesicles isolated from the same types of mineralizing cells have distinct lipid and protein composition as well as functions. These findings support the view that matrix vesicles and media vesicles released by mineralizing cells have different functions in mineralized tissues due to their location, which is anchored to the extracellular matrix versus free-floating.

Keywords: biomineralization; cell-cell communication; collagenase; matrix vesicles; media extracellular vesicles; ultracentrifugation.

Publication types

Review

MeSH terms

Calcinosis*
Chondrocytes
Extracellular Matrix
Extracellular Vesicles*
Humans
Hypertrophy

Grants and funding

This work was supported by the Italian Ministry for Research [2022 Program for Research Projects of National Interest (PRIN), Grant # 20229RMZZW]; the ERA-CVD/MICROEXPLORATION/4/2018 grant from the National Centre for Research and Development, Poland; the Hubert Curien Partnership Programme POLONIUM # PPN/BFR/2020/1/00056 for years 2021/2022 co-financed by the Polish National Agency for Academic Exchange and the Ministry of Foreign Affairs and International Development of France; the statutory funds of the Nencki Institute of Experimental Biology, Polish Academy of Sciences; France-UK Hubert Curien Partnership Programme ALLIANCE; the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grants 2019/08568-2); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 305426/2021-4); the Engineering and Physical Sciences Research Council [EP/V062425/1] and the Academy of Medical Sciences [SBF004\1090].