Significance of Gastrokine-1 Polymorphism Rs4254535 as a Prognostic Marker and its Association with Clinical Characteristics in Chinese Lung Cancer Patients

Zixiu Zou; Chang Xu; Zhengxing Li; Yajun Yang; Yutao Li; Zhenyu Sun; Qiang Li; Miao Li; Yuxin Chen; Gengxi Jiang; Man Xiao; Shicheng Guo; Yi Wang; Haijian Wang; Fan Xia; Yan Shang; Junjie Wu

doi:10.7150/ijms.90145

Significance of Gastrokine-1 Polymorphism Rs4254535 as a Prognostic Marker and its Association with Clinical Characteristics in Chinese Lung Cancer Patients

Int J Med Sci. 2024 Jan 1;21(3):474-482. doi: 10.7150/ijms.90145. eCollection 2024.

Authors

Zixiu Zou¹, Chang Xu², Zhengxing Li³, Yajun Yang¹, Yutao Li⁴, Zhenyu Sun⁵, Qiang Li⁶, Miao Li⁷, Yuxin Chen⁸, Gengxi Jiang⁹, Man Xiao¹⁰, Shicheng Guo⁴, Yi Wang⁴, Haijian Wang⁴, Fan Xia¹¹, Yan Shang^{12

13}, Junjie Wu^{7

14

4}

Affiliations

¹ School of Life Sciences, Fudan University, Shanghai, 200438, China.
² Clinical College of Xiangnan University, Chenzhou, 423000, China.
³ Department of Surgery, Navy Military Medical University Affiliated to Changhai Hospital, Shanghai, China.
⁴ State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics & Development, School of Life Sciences, Fudan University, Shanghai, 200438, China.
⁵ School of Basic Medicine, Navy Military Medical University, Shanghai, 200433, China.
⁶ Department of Respiratory and Critical Care Medicine, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.
⁷ Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
⁸ Nanjing Medical University, The Fourth Clinical Medical College, Nanjing, 211166, China.
⁹ Department of Thoracic Surgery, Navy Military Medical University Affiliated Changhai Hospital, Shanghai, 200433, China.
¹⁰ Department of Biochemistry and Molecular Biology, Hainan Medical University, Haikou, 571199, China.
¹¹ Department of Respiratory Disease, Navy 905 Hospital, Shanghai, 200235, China.
¹² Department of Respiratory and Critical Care Medicine, Shanghai Changhai Hospital, the First Afliated Hospital of Naval Medical University, Shanghai 200433, China.
¹³ Department of General Medicine, the First Afliated Hospital of Naval Medical University, Shanghai 200433, China.
¹⁴ Department of Pulmonary and Critical Care Medicine, Shanghai Geriatric Medical Center, Shanghai, 200032, China.

Abstract

Background: The single nucleotide polymorphism (SNP) of Gastrokine-1 (GKN1) is associated with lung cancer but its association with prognosis is not clear. Methods: Genomic DNA was extracted from the blood samples of 888 patients with lung cancer. The association between GKN1 polymorphism rs4254535 and prognostic was analyzed by the Kaplan-Meier (KM) method, Log-rank test, and Cox proportional hazards model. Results: In females and patients diagnosed with late-stage lung cancer, the CC genotype (CC vs TT, adjusted odds ratio [HR] = 0.57, 95% Confidence Interval [CI]: 0.33-0.99, P = 0.045; HR = 0.66, 95% CI: 0.48-0.92, P = 0.014) and recessive CC genotype (CC vs TT + TC, HR = 0.55, 95% CI: 0.32-0.94, P = 0.028; HR = 0.64, 95% CI: 0.47-0.89, P = 0.006) of rs4254535 conferred a better prognosis, compared with the TT and TT + TC genotype. Rs4254535 dominate TC + CC genotype, recessive CC genotype, and C allele who were adenocarcinoma patients had a significantly better prognosis. The recessive CC genotype of non-smoking patients has a better prognosis, compared to the TT + TC genotype. Additionally, in the dominant TT + TC genotype and C allele, no family history patients had a significantly better prognosis, compared to the TT genotype. Conclusion: For lung cancer patients, GKN1 polymorphism rs4254535 may be a protective genetic marker and predicts the prognosis of lung cancer patients.

Keywords: GKN1; lung cancer; prognosis; rs4254535; single nucleotide polymorphism.

MeSH terms

China
Female
Genotype
Humans
Lung Neoplasms* / genetics
Peptide Hormones*
Polymorphism, Single Nucleotide / genetics
Prognosis

Substances

GKN1 protein, human
Peptide Hormones