A FRET based ultrasensitive fluorescent aptasensor for 6'-sialyllactose detection

Jinri Chen; Yuting Zhang; Xiaoli Wang; Fuhou Li; Shaojie Wu; Weixia Wang; Nandi Zhou

doi:10.1016/j.ab.2024.115462

A FRET based ultrasensitive fluorescent aptasensor for 6'-sialyllactose detection

Anal Biochem. 2024 May:688:115462. doi: 10.1016/j.ab.2024.115462. Epub 2024 Jan 19.

Authors

Jinri Chen¹, Yuting Zhang², Xiaoli Wang², Fuhou Li³, Shaojie Wu³, Weixia Wang³, Nandi Zhou⁴

Affiliations

¹ The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Haizhou, 222005, China; State Key Laboratory of Genetic Engineering, MOE Engineering Research Centre of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
² The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
³ Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Haizhou, 222005, China.
⁴ The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. Electronic address: zhounandi@jiangnan.edu.cn.

PMID: 38246433
DOI: 10.1016/j.ab.2024.115462

Abstract

As a kind of human milk oligosaccharide, 6'-sialyllactose (6'-SL) plays an important role in promoting infant brain development and improving infant immunity. The content of 6'-SL in infant formula milk powder is thus one of the important nutritional indexes. Since the lacking of efficient and rapid detection methods for 6'-SL, it is of great significance to develop specific recognition elements and establish fast and sensitive detection methods for 6'-SL. Herein, using 6'-SL specific aptamer as the recognition element, catalytic hairpin assembly as the signal amplification technology and quantum dots as the signal label, a fluorescence biosensor based on fluorescence resonance energy transfer (FRET) was constructed for ultra-sensitive detection of 6'-SL. The detection limit of this FRET-based fluorescent biosensor is 0.3 nM, and it has some outstanding characteristics such as high signal-to-noise ratio, low time-consuming, simplicity and high efficiency in the actual sample detection. Therefore, it has broad application prospect in 6'-SL detection.

Keywords: 6′-Sialyllactose; Aptamer; Aptasensor; FRET; Fluorescent biosensor.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques* / methods
Coloring Agents
Fluorescence Resonance Energy Transfer / methods
Humans
Lactose / analogs & derivatives*
Limit of Detection
Milk, Human
Quantum Dots*

Substances

6'-sialyllactose
Aptamers, Nucleotide
Coloring Agents
Lactose