Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum

Jie Hu; Panpan He; Fang Zhao; Weihao Lin; Chenlong Xue; Jinna Chen; Zhiguang Yu; Yang Ran; Yue Meng; Xin Hong; Perry Ping Shum; Liyang Shao

doi:10.1016/j.talanta.2024.125625

Magnetic microspheres enhanced peanut structure cascaded lasso shaped fiber laser biosensor for cancer marker-CEACAM5 detection in serum

Talanta. 2024 May 1:271:125625. doi: 10.1016/j.talanta.2024.125625. Epub 2024 Jan 5.

Authors

Jie Hu¹, Panpan He², Fang Zhao³, Weihao Lin⁴, Chenlong Xue⁵, Jinna Chen⁶, Zhiguang Yu⁷, Yang Ran⁸, Yue Meng⁹, Xin Hong¹⁰, Perry Ping Shum¹¹, Liyang Shao¹²

Affiliations

¹ Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: 12031313@mail.sustech.edu.cn.
² Medcaptain Medical Technology Co., Ltd., Shenzhen, 518055, China. Electronic address: fiona_he2026@126.com.
³ Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: 12031197@mail.sustech.edu.cn.
⁴ Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: 11510630@mail.sustech.edu.cn.
⁵ Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: 12132154@mail.sustech.edu.cn.
⁶ Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: chenjn@sustech.edu.cn.
⁷ Medcaptain Medical Technology Co., Ltd., Shenzhen, 518055, China. Electronic address: george.yu@medcaptain.com.
⁸ Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, Guangzhou, 510632, China. Electronic address: tranyang@jnu.edu.cn.
⁹ Department of Clinical Laboratory, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 511436, China. Electronic address: mengyue@gdph.org.cn.
¹⁰ Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, China; Key University Laboratory of Metabolism and Health of Guangdong, Southern University of Science and Technology, Shenzhen, 518055, Guangdong, China. Electronic address: hongx@sustech.edu.cn.
¹¹ Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: shenp@sustech.edu.cn.
¹² Department of Electronic and Electrical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: shaoly@sustech.edu.cn.

PMID: 38244308
DOI: 10.1016/j.talanta.2024.125625

Abstract

The detection of trace cancer markers in body fluids such as blood/serum is crucial for cancer diseases screening and treatment, which requires high sensitivity and specificity of biosensors. In this study, a peanut structure cascaded lasso (PSCL) shaped fiber sensing probe based on fiber laser demodulation method was proposed to specifically detect the carcinoembryonic antigen related cell adhesion molecules 5 (CEACAM5) protein in serum. Thanks for the narrow linewidth and high signal-to-noise ratio (SNR) of the laser spectrum, it is easier to distinguish small spectral changes than interference spectrum. Adding the antibody modified magnetic microspheres (MMS) to form the sandwich structure of "antibody-antigen-antibody-MMS", and amplified the response caused by biomolecular binding. The limit of detection (LOD) for CEACAM5 in buffer could reach 0.11 ng/mL. Considering the common threshold of 5 ng/mL for CEA during medical screening and the cut off limit of 2.5 ng/mL for some kits, the LOD of proposed biosensor meets the actual needs. Human serum samples from a hospital were used to validate the real sensing capability of proposed biosensor. The deviation between the measured value in various serum samples and the clinical value ranged from 1.9 to 9.8 %. This sensing scheme holds great potential to serve as a point of care testing (POCT) device and extend to more biosensing applications.

Keywords: CEACAM5 protein; Fiber laser; Human serum; Magnetic microsphere; Optical fiber biosensor.

MeSH terms

Arachis*
Carcinoembryonic Antigen
Cell Adhesion Molecules
GPI-Linked Proteins
Humans
Lasers
Magnetic Phenomena
Microspheres
Neoplasms*

Substances

Cell Adhesion Molecules
CEACAM5 protein, human
Carcinoembryonic Antigen
GPI-Linked Proteins