Unlocking the potential of co-application of steel slag and biochar in mitigation of arsenic-induced oxidative stress by modulating antioxidant and glyoxalase system in Abelmoschus esculentus L

Abstract

This study investigates our hypothesis that how effect of arsenic stress on okra (Abelmoschus esculentus L.) can be alleviated through the use of waste materials such as steel slag (SS) and corncob biochar (BC). Different growth variables, biochemical parameters, oxidative stress markers, enzymatic and non-enzymatic antioxidants and glyoxylase enzyme activities were assessed. When okra was exposed to As, there was a noticeable decrease in seedling length, biomass, relative water content, various biochemical attributes, however, electrolyte leakage and lipid peroxidation in okra were enhanced. The supplementation of SS and BC-either individually or in combination-improved the growth parameters and reduced oxidative stress markers. Application of SS and BC also lowered As accumulation in roots and shoots of okra mitigating adverse effects of As exposure. Additionally, the activities of antioxidant and glyoxalase enzyme increased when SS and BC were present, concurrently reducing methylglyoxal content. Arsenic-induced stress led to oxidative damage, an enhancement in both enzymatic and non-enzymatic antioxidants, induced the synthesis of thiol and phytochelatins in roots and shoots. These may play a vital function in alleviating oxidative stress induced by As. Superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase activities were significantly enhanced in As-treated plants. These enhancement were further amplified when SS and BC were amended to As-treated okra. Therefore, synergistic application of SS and BC effectively protects okra against oxidative stress induced by As by increasing both antioxidant defense and glyoxalase systems. Both SS, an industrial byproduct, and BC, generated from agricultural waste, are cost-effective, environmentally friendly, safe, and non-toxic materials which can be used for crop production in As contaminated soil.

Keywords: Antioxidant defense; Environmental contaminants; Industrial wastes; Metal chelation; Oxidative stress; Remediation techniques.