Facile and green synthesis of chlorophyll-derived multi-color fluorescent carbonized polymer dots and their use for sensitive detection of hemin

Tianjiao Liu; Deming Dong; Yingyi Meng; Haijun Chen; Chunyue Liu; Zihan Qi; Anfeng Li; Yang Ning

doi:10.1016/j.saa.2024.123841

Facile and green synthesis of chlorophyll-derived multi-color fluorescent carbonized polymer dots and their use for sensitive detection of hemin

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Apr 5:310:123841. doi: 10.1016/j.saa.2024.123841. Epub 2024 Jan 14.

Authors

Tianjiao Liu¹, Deming Dong², Yingyi Meng³, Haijun Chen⁴, Chunyue Liu⁵, Zihan Qi⁶, Anfeng Li⁷, Yang Ning⁸

Affiliations

¹ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: liutj20@mails.jlu.edu.cn.
² Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: dmdong@jlu.edu.cn.
³ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: mengyy22@mails.jlu.edu.cn.
⁴ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: chj20@mails.jlu.edu.cn.
⁵ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: cyliu23@mails.jlu.edu.cn.
⁶ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: qizh18@mails.jlu.edu.cn.
⁷ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: liaf17@mails.jlu.edu.cn.
⁸ Key Lab of Groundwater Resources and Environment Ministry of Education, College of New Energy and Environment, Jilin University, 2699 Qianjin Avenue, Changchun 130012, PR China. Electronic address: ningyang@jlu.edu.cn.

PMID: 38241933
DOI: 10.1016/j.saa.2024.123841

Abstract

Due to the very important role in physiological process, a simple and sensitive hemin detection method is necessarily required. Biomass-based carbonized polymer dots (CPDs) have been widely studied especially as fluorescence probe owing to the advantages of low toxicity and the variety of fluorescence color, yet there are still challenges in developing their multi-color emission property from the same raw materials. In this work, red, white and blue emissive CPDs derived from chlorophyll have been synthesized via hydrothermal method. Then white-emitted CPDs (white-CPDs) with the Commission International d'Eclairage (CIE) coordinates at (0.34, 0.32) were used to develop a fluorescence quenched sensing system for hemin determination. There is a good linear relationship between (F₀-F)/F₀ and concentration of hemin in the range of 0.1-0.95 μM with a detection limit of 0.043 μM, and the quenching mechanism was considered to be caused by inner filter effect (IFE). Moreover, it has been successfully used for hemin detection in serum and also for visual determination, which indicating great potential in applications of disease diagnoses and trace identification.

Keywords: Carbonized polymer dots; Chlorophyll; Hemin; Inner filter effect; White-emission.

MeSH terms

Carbon
Fluorescent Dyes
Hemin
Polymers
Quantum Dots*
Spectrometry, Fluorescence / methods

Substances

Hemin
Polymers
Fluorescent Dyes
Carbon