Small nucleolar RNA Snora73 promotes psoriasis progression by sponging miR-3074-5p and regulating PBX1 expression

Funct Integr Genomics. 2024 Jan 19;24(1):15. doi: 10.1007/s10142-024-01300-7.

Abstract

Chronic psoriasis is a kind of immune-mediated skin illness and the underlying molecular mechanisms of pathogenesis remain incompletely understood. Here, we used small RNA microarray assays to scan the differential expressed RNAs in psoriasis patient samples. The downstream miRNAs and its targets were predicted using bioinformatics analysis from online bases and confirmed using fluorescence in situ hybridization and dual‑luciferase report gene assay. Cell ability of proliferation and migration were detected using CCK-8 and transwell assays. The results showed that a new snoRNA Snora73 was upregulated in psoriasis patient samples. Overexpression of Snora73 significantly increased psoriasis cells viability and migration, while knockdown of Snora73 got the opposite results. Mechanistically, our results showed that Snora73 acted as a sponge for miR-3074-5p and PBX1 is a direct target of miR-3074-5p in psoriasis cells. Furthermore, miR-3074-5p suppressed psoriasis cell proliferation and migration, while PBX1 promoted cell proliferation and migration in psoriasis. Collectively, these findings reveal a crucial role of Snora73 in progression of psoriasis through miR-3074-5p/PBX1 signaling pathway and suggest a potential therapeutic strategy.

Keywords: PBX1; Psoriasis; Snora73; miR-3074-5p; snoRNA.

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • MicroRNAs* / genetics
  • Pre-B-Cell Leukemia Transcription Factor 1* / genetics
  • Psoriasis* / genetics
  • RNA, Long Noncoding* / genetics
  • RNA, Small Nucleolar* / genetics

Substances

  • MicroRNAs
  • MIRN3074 microRNA, human
  • RNA, Long Noncoding
  • RNA, Small Nucleolar
  • PBX1 protein, human
  • Pre-B-Cell Leukemia Transcription Factor 1

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