Preclinical Mouse Metastatic Model Established Through Induced Lung Metastases

Katja Uršič Valentinuzzi; Gregor Serša; Urška Kamenšek

doi:10.1007/978-1-0716-3714-2_8

Preclinical Mouse Metastatic Model Established Through Induced Lung Metastases

Methods Mol Biol. 2024:2773:77-86. doi: 10.1007/978-1-0716-3714-2_8.

Authors

Katja Uršič Valentinuzzi^{1

2}, Gregor Serša^{1

3}, Urška Kamenšek^{4

5}

Affiliations

¹ Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia.
² Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
³ Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
⁴ Department of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, Slovenia. Ukamensek@onko-i.si.
⁵ Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia. Ukamensek@onko-i.si.

PMID: 38236538
DOI: 10.1007/978-1-0716-3714-2_8

Abstract

Metastatic disease is the major cause of cancer death, and the lung is one of the most common sites of cancer metastases. To investigate systemic antitumor effects or protective potential of local therapies, mouse models with induced metastases are indispensable in preclinical cancer research. Here, we describe the protocol for the metastatic mouse model established through induced 4T1 mammary carcinoma metastases. With minor prior optimization, it can be applied to other tumor cell lines of interest.

Keywords: Cancer; Lung colonization assay; Lungs; Metastases; Mouse models.

MeSH terms

Animals
Cell Line, Tumor
Disease Models, Animal
Lung Neoplasms*
Mice
Thorax