LncRNA PSMB8-AS1 increases glioma malignancy via the miR-382-3p/BCAT1 axis

Haibo Liu; Jie Zhang; Jiamin Li; Xiaoying Cao; Kai Yu; Xun Xia; Zongxi Li; Fengbo Wang

doi:10.1016/j.tranon.2023.101806

LncRNA PSMB8-AS1 increases glioma malignancy via the miR-382-3p/BCAT1 axis

Transl Oncol. 2024 Jan:39:101806. doi: 10.1016/j.tranon.2023.101806. Epub 2023 Oct 28.

Authors

Haibo Liu¹, Jie Zhang², Jiamin Li³, Xiaoying Cao², Kai Yu², Xun Xia², Zongxi Li², Fengbo Wang⁴

Affiliations

¹ Department of Neurosurgery, Pengzhou People's Hospital, Chengdu 610500, Sichuan, China; Department of Neurosurgery, Pengzhou Second People's Hospital, Chengdu 610500, Sichuan, China; Department of Neurosurgery, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, Sichuan, China.
² Department of Neurosurgery, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, Sichuan, China.
³ Department of Critical Care Medicine, Xindu District People's Hospital of Chengdu, Chengdu 610500, Sichuan, China.
⁴ Department of Rehabilitation, The First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, Sichuan, China. Electronic address: fengbowang2@163.com.

Abstract

Background: This study aimed to investigate the specific roles of the long non-coding RNA (lncRNA) proteasome 20S subunit beta 8 (PSMB8)-antisense RNA 1 (AS1)/microRNA (miR)-382-3p/branched-chain amino acid transaminase 1 (BCAT1) interaction network in gliomas.

Methods: Western blotting and quantitative reverse transcription-polymerase chain reaction were performed to assess the expression levels of lncRNA PSMB8-AS1, BCAT1, and miR-382-3p. Moreover, the cell proliferation, migration, and apoptosis were assessed using the cell counting kit-8, Transwell, and caspase-3 activity assays, respectively. The biological role of lncRNA PSMB8-AS1 in glioma was investigated in vivo using a xenograft mouse model. Additionally, the associations among lncRNA PSMB8-AS1, miR-382-3p, and BCAT1 were analyzed using dual-luciferase and RNA immunoprecipitation assays and bioinformatics analyses.

Results: Glioma cell lines and tissues exhibited overexpression of lncRNA PSMB8-AS1 and BCAT1 and low expression of miR-382-3p. Knockdown of PSMB8-AS1 remarkably repressed the tumor growth in vivo and the migration and proliferation of glioma cells in vitro. In contrast, knockdown of lncRNA PSMB8-AS1 increased the cell apoptosis. Mechanistically, PSMB8-AS1 directly targeted miR-382-3p. By sponging miR-382-3p, lncRNA PSMB8-AS1 stimulated the migration and proliferation of glioma cells and suppressed their apoptosis. Additionally, miR-382-3p directly targeted BCAT1. Inhibition of miR-382-3p reversed the antitumor effects of BCAT1 silencing on glioma progression.

Conclusion: Our study revealed that lncRNA PSMB8-AS1 aggravated glioma malignancy by enhancing BCAT1 expression after competitively binding to miR-382-3p. Therefore, lncRNA PSMB8-AS1 may be a potential biomarker and therapeutic target for glioma treatment.

Keywords: BCAT1; Glioma; Proliferation; lncRNA PSMB8-AS1; miR-382-3p.