Integrating porphyrin-based nanoporous organic polymers with electrochemical aptasensors for ultratrace detection of kanamycin

Guanghui Tian; Feng Guo; Chuanbin Fan; Zi-Ao Zong; Junli Wang; Zhuorigebatu Tegudeer; Wen-Yang Gao

doi:10.1007/s00604-024-06180-z

Integrating porphyrin-based nanoporous organic polymers with electrochemical aptasensors for ultratrace detection of kanamycin

Mikrochim Acta. 2024 Jan 17;191(2):100. doi: 10.1007/s00604-024-06180-z.

Authors

Guanghui Tian¹, Feng Guo², Chuanbin Fan¹, Zi-Ao Zong¹, Junli Wang¹, Zhuorigebatu Tegudeer³, Wen-Yang Gao⁴

Affiliations

¹ School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.
² School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. guofeng1510@yeah.net.
³ Department of Chemistry and Biochemistry, Ohio University, Athens, OH, 45701, USA.
⁴ Department of Chemistry and Biochemistry, Ohio University, Athens, OH, 45701, USA. gaow@ohio.edu.

Abstract

The synthesis and characterization of two new porphyrin-based porous organic polymers (POPs) via Sonogashira cross-coupling reaction and leverage the two obtained POPs is reported for the fabrication of electrochemical aptasensors to detect kanamycin at an ultratrace level. The resultant electrochemical aptasensor demonstrates a high linear relationship with the logarithmic value of kanamycin concentration in the range 5 × 10^-5-5 μg/L with the limit of detection of 17.6 pg/L or 36.3 fM. During the analysis of real samples from milk and river, a relative standard deviation of less than 4.39%, and good recovery values in the range 97.0-105% were obtained.

Keywords: Electrochemical aptasensors; Electrochemical impedance spectroscopy; Kanamycin; Porous organic polymers; Ultratrace detection.

Abstract

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