Engineering tunable dual peptide hybrid coatings promote osseointegration of implants

Zeyu Shou; Zhibiao Bai; Han Zhou; Yizhe Shen; Xiaojing Huang; Hongming Meng; Chenwei Xu; Shaohao Wu; Na Li; Chun Chen

doi:10.1016/j.mtbio.2023.100921

Engineering tunable dual peptide hybrid coatings promote osseointegration of implants

Mater Today Bio. 2023 Dec 16:24:100921. doi: 10.1016/j.mtbio.2023.100921. eCollection 2024 Feb.

Authors

Zeyu Shou^{1

2}, Zhibiao Bai¹, Han Zhou¹, Yizhe Shen¹, Xiaojing Huang¹, Hongming Meng¹, Chenwei Xu¹, Shaohao Wu¹, Na Li², Chun Chen^{1

3

4}

Affiliations

¹ Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, People's Republic of China.
² Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, People's Republic of China.
³ Key Laboratory of Intelligent Treatment and Life Support for Critical Diseases of Zhejiang Province, Wenzhou, 325000, Zhejiang, People's Republic of China.
⁴ Zhejiang Engineering Research Center for Hospital Emergency and Process Digitization, Wenzhou, Zhejiang, 325000, People's Republic of China.

Abstract

Utilizing complementary bioactive peptides is a promising surface engineering strategy for bone regeneration on osteogenesis. In this study, we designed block peptides, (Lysine)₆-capped RGD (K₆-(linker-RGD)₃) and OGP (K₆-linker-(YGFGG)₂), which were mildly grafted onto PC/Fe-MPNs through supramolecular interactions between K₆ and PC residues on the MPNs surface to form a dual peptide coating, named PC/Fe@K₆-RGD/OGP. The properties of the block peptides coating, including mechanics, hydrophilicity, chemical composition, etc., were detailly characterized by various techniques (ellipsometry, quartz crystal microbalance, X-ray photoelectron spectroscopy, water contact angle, scanning electronic microscopy and atomic force microscopy). Importantly, the RGD/OGP ratio can be well adjusted, which allowed optimizing the RGD/OGP ratio to endow significantly enhanced osteogenic activity of MC3T3-E1 cells through the Wnt/β-catenin pathway, while also promoting cell adhesion, immune regulation, inhibiting osteoclast differentiation and oxidative stress reduction. In vivo, the optimized RGD/OGP coatings promoted bone regeneration and osseointegration around implants in rats with bone defects. In conclusion, rationally designed PC/Fe@K₆-RGD/OGP coating integrated RGD and OGP bioactivities, providing a convenient approach to enhance bioinert implant surfaces for bone regeneration.

Keywords: Block peptides; Implants; Metal-phenolic networks; Osseointegration; Surface modification.