Effects of Metabolites of Lactobacillus casei on Expression and Neutralization of Shiga Toxin by Enterohemorrhagic Escherichia coli

Arpita Aditya; Zajeba Tabashsum; Zabdiel Alvarado Martinez; Debabrata Biswas

doi:10.1007/s12602-024-10212-8

Effects of Metabolites of Lactobacillus casei on Expression and Neutralization of Shiga Toxin by Enterohemorrhagic Escherichia coli

Probiotics Antimicrob Proteins. 2024 Jan 15. doi: 10.1007/s12602-024-10212-8. Online ahead of print.

Authors

Arpita Aditya¹, Zajeba Tabashsum², Zabdiel Alvarado Martinez², Debabrata Biswas^{3

4

5}

Affiliations

¹ Department of Animal Sciences, University of Maryland, College Park, MD, 20742, USA.
² Biological Sciences Program, University of Maryland, College Park, MD, 20742, USA.
³ Department of Animal Sciences, University of Maryland, College Park, MD, 20742, USA. dbiswas@umd.edu.
⁴ Biological Sciences Program, University of Maryland, College Park, MD, 20742, USA. dbiswas@umd.edu.
⁵ Centre for Food Safety and Security Systems, University of Maryland, College Park, MD, 20742, USA. dbiswas@umd.edu.

PMID: 38224447
DOI: 10.1007/s12602-024-10212-8

Abstract

Shiga toxin (stx), produced by enterohemorrhagic Escherichia coli (EHEC) or Shigella, causes hemolytic uremic syndrome (HUS) in humans. EHEC-mediated illnesses are recommended to treat by immune supportive strategies, instead of antibiotic therapy. Widely used probiotic Lactobacillus casei produces many bioactive metabolites, i.e., conjugated linoleic acids (CLAs) which have potential to educate host immunity and control EHEC growth and expression of its virulence genes. In this study, it was found that total metabolites of L. casei exerted a protective effect on Gb₃ receptor containing mammalian cells against stx exposure.

Keywords: Conjugated linoleic acid; EHEC; Metabolites; Probiotics; Shiga toxin.

Grants and funding

20185110628809/USDA-National Institute of Food and Agriculture