A ratiomectic aptasensor with enhanced signals based on peroxidase-like enzymes and NH2-MIL-101@MoS2 for trace detection of deoxynivalenol in traditional Chinese herbs

Food Chem. 2024 May 30:441:138381. doi: 10.1016/j.foodchem.2024.138381. Epub 2024 Jan 8.

Abstract

The accumulation of the deoxynivalenol (DON) in the human body poses a significant health risk that is often overlooked, and we urgently need an ultra-sensitive rapid detection platform. Due to the porosity of NH2-MIL-101@MoS2, an increased loading of toluidine blue (TB) serves to create a signal reference. Cobalt@carbon (CoC) derived from metal organic frameworks was combined with NH2-MIL-101(NH2-MIL-101@CoC) to form an enzyme-free Nanoprobe (Apt-pro) with significant catalytic properties. The ratio (IBQ /ITB) was changed by varying the electrochemical signal of benzoquinone (BQ) (IBQ) and the amount of TB deposition (ITB). This aptasensor was successfully applied to detect DON in malt and peach seed, which exhibited a great linear range from 1 fg/mL to 10 ng/mL and low detection limit of 0.31 fg/mL for DON.

Keywords: Aptasensor; Dual signal; Electrochemical detection; Metal organic frameworks; Peroxidase-like enzymes; Traditional Chinese herbs.

MeSH terms

  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques*
  • Coloring Agents
  • Electrochemical Techniques
  • Humans
  • Limit of Detection
  • Metal Nanoparticles* / chemistry
  • Metal-Organic Frameworks* / chemistry
  • Molybdenum
  • Peroxidase / chemistry
  • Trichothecenes*

Substances

  • MIL-101
  • Metal-Organic Frameworks
  • Peroxidase
  • Molybdenum
  • deoxynivalenol
  • Coloring Agents
  • Aptamers, Nucleotide
  • Trichothecenes