Role of microRNA, bta-miR-375 in Immune Sturdiness of Vechur: The Native Cattle Breed of Kerala, India

Divya P D; Shynu M; Jayavardhanan K K; Uma R; Aravindakshan T V; Radhika G; Sameer Kumar V B; Muhasin Asaf; Renjith Sebastian

doi:10.1016/j.heliyon.2023.e22683

Role of microRNA, bta-miR-375 in Immune Sturdiness of Vechur: The Native Cattle Breed of Kerala, India

Heliyon. 2023 Nov 22;9(12):e22683. doi: 10.1016/j.heliyon.2023.e22683. eCollection 2023 Dec.

Authors

Divya P D¹, Shynu M², Jayavardhanan K K², Uma R², Aravindakshan T V³, Radhika G³, Sameer Kumar V B⁴, Muhasin Asaf⁵, Renjith Sebastian¹

Affiliations

¹ Department of Veterinary Biochemistry, CVAS, Pookode, Kerala Veterinary and Animal Sciences University, Kerala, India.
² Department of Veterinary Biochemistry, CVAS, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
³ CASAGB, CVAS, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
⁴ Department of Genomic Science, Central University of Kerala, Kasaragod, India.
⁵ Department of Animal Breeding and Genetics, CVAS, Pookode, Kerala Veterinary and Animal Sciences University, Kerala, India.

Abstract

In the present study, next generation sequencing was employed to identify and explore the differential expression profiles of microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) of crossbred (B. taurus x B. indicus) and Vechur (B. indicus) cattle in response to the bacterial endotoxin-lipopolysaccharide (LPS). The PBMCs from adult apparently healthy female crossbred cows and Vechur cattle, a native cattle breed of Kerala, India were stimulated with 10 μg/mL of LPS for 6 h. Among the differentially expressed miRNAs, the expression of 13 miRNAs showed statistically significant up regulation while, significant decrease in the expression of 15 miRNAs was noticed in LPS treated PBMCs of Vechur cattle compared to crossbred cows. The expression profiling of miRNA, bta-miR-375, expression of which was found to be significantly down regulated in LPS treated PBMCs of Vechur cattle with respect to crossbred cattle by the NGS studies, is presented in the present manuscript. The decrease in expression of bta-miR-375 noticed by NGS was in accordance with the results of quantitative real time PCR assay. Functional gene enrichment analysis and pathway analysis revealed significant enrichment of predicted targets of bta-miR-375 in many immune related and cell signalling mechanisms. In addition, over representation of targets of bta-miR-375 was also noticed in pathogenesis of many of the bovine diseases. The study could also identify differences in the expression of cytokines, viz. Tumour Necrosis Factor Alpha (TNFα), Interleukin 4 (IL-4) and Interferon-γ (IFNγ) between LPS treated and untreated PBMCs of crossbred and Vechur cattle.

Keywords: Cytokines; Lipopolysaccharide; Peripheral blood mononuclear cells; Vechur cattle; microRNA.