Membrane technology for the purification of RNA and DNA therapeutics

Amin Javidanbardan; Kevork Oliver Messerian; Andrew L Zydney

doi:10.1016/j.tibtech.2023.11.016

Membrane technology for the purification of RNA and DNA therapeutics

Trends Biotechnol. 2024 Jan 11:S0167-7799(23)00338-4. doi: 10.1016/j.tibtech.2023.11.016. Online ahead of print.

Authors

Amin Javidanbardan¹, Kevork Oliver Messerian¹, Andrew L Zydney²

Affiliations

¹ Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
² Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address: zydney@engr.psu.edu.

PMID: 38212210
DOI: 10.1016/j.tibtech.2023.11.016

Abstract

Nucleic acid therapeutics have the potential to revolutionize the biopharmaceutical industry, providing highly effective vaccines and novel treatments for cancers and genetic disorders. The successful commercialization of these therapeutics will require development of manufacturing strategies specifically tailored to the purification of nucleic acids. Membrane technologies already play a critical role in the downstream processing of nucleic acid therapeutics, ranging from clarification to concentration to selective purification. This review provides an overview of how membrane systems are currently used for nucleic acid purification, while highlighting areas of future need and opportunity, including adoption of membranes in continuous bioprocessing.

Keywords: DNA; mRNA; membrane; ultrafiltration.

Publication types

Review