An integrative multi-omics approach aimed to gain insight on the effect of composition, style, yeast, and wheat species on wheat craft beer flavour

Abstract

This study was aimed to unravel the effect of raw materials (barley and wheat), wheat concentration (0, 25, 40, and 100 %), wheat species (common and durum), beer style (Blanche and Weiss), and yeast (US-05 and WB-06) on the chemical composition, volatiles, and sensory profile of wheat craft beers by using a multivariate statistical approach. Beer samples were analysed for their composition, volatiles and sensory profile and data were processed using unsupervised multivariate analyses, PLS regression and a multi-omics approach using multi-block PLS-DA. Multi-block variable sparsification was used as an embedded dimension reduction step. The adopted multi-omics approach permitted to correctly classify beers with different styles and wheat concentration, and to accurate classify (95 % accuracy) beers according to yeast type. Wheat species was of lower importance since it permitted a classification with 49 % accuracy which increased to 74 % in Blanche beers, thus suggesting that malting flattened differences determined by wheat species.

Keywords: Beer formulation; Multi-omics; Multivariate statistics; Sensory analysis; Volatile organic compounds; Wheat beer.