Structure of the O-polysaccharide from the moderately halophilic bacterium Halomonas fontilapidosi KR26

Abstract

Lipopolysaccharide was obtained from the aerobic moderately halophilic bacterium Halomonas fontilapidosi KR26. The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide and was examined by chemical methods and by ¹H and ¹³C NMR spectroscopy, including ¹H,¹H COSY, TOCSY, ROESY, and ¹H,¹³C HSQC, and HMBC experiments. The following structure of the linear tetrasaccharide repeating unit was deduced. →2)-α-l-Rhap-(1→2)-α-l-Rhap-(1→3)-α-l-Rhap-(1→3)-β-d-Galp-(1→.

Keywords: Bacterial polysaccharide structure; Halomonas fontilapidosi; Lipopolysaccharide; O-specific polysaccharide.