In vitro evaluation of enterococcus faecalis growth in different conditions on dentinal substrate

Wajih Hage; Dolla Karam Sarkis; Mireille Kallassy; May Mallah; Carla Zogheib

doi:10.1080/26415275.2023.2287668

In vitro evaluation of enterococcus faecalis growth in different conditions on dentinal substrate

Biomater Investig Dent. 2023 Dec 20;10(1):2287668. doi: 10.1080/26415275.2023.2287668. eCollection 2023.

Authors

Wajih Hage¹, Dolla Karam Sarkis², Mireille Kallassy³, May Mallah², Carla Zogheib¹

Affiliations

¹ Departement of Endodontics, Saint Joseph University, Beirut, Lebanon.
² Departement of Microbiology, Saint Joseph University, Beirut, Lebanon.
³ Departement of Sciences, Saint Joseph University, Beirut, Lebanon.

Abstract

The aim of this study was to find the best growth conditions of Enterococcus faecalis on a dentinal substrate in order to be used for the development of a complex multispecies endodontic biofilm. Fifty two single rooted extracted human teeth and fifty two dentinal disks were mechanically prepared, sterilized, inoculated with Enterococcus faecalis and divided randomly into 8 groups where the substrate, the inoculation technique, the medium type, and the pre-treatment with collagen type I was varied. Bacterial count was evaluated and colonies were counted and confirmed by colony morphology observation on blood agar and Gram staining at 3,7, 14, 21, and 28 days. On day 14 of the culture, the bacterial count showed the highest values in all groups. Root canals and Type 1 collagen pre-treatment and glucose proved to have significant positive effects on the bacterial count compared to dentinal disks and BHI media only. The increase in bacterial count found with the direct inoculation technique was not significantly different from that of the indirect technique.