The high demand for flax as a nutritious edible oil source combined with increasingly restrictive import regulations for oilseeds mandates the exploration of novel quantity and quality assessment methods. One pervasive issue that compromises the viability of flaxseeds is the mechanical damage to the seeds during harvest and post-harvest handling. Currently, mechanical damage in flax is assessed via visual inspection, a time-consuming, subjective, and insufficiently precise process. This study explores the potential of hyperspectral imaging (HSI) combined with chemometrics as a novel, rapid, and non-destructive method to characterize mechanical damage in flaxseeds and assess how mechanical stresses impact the germination of seeds. Flaxseed samples at three different moisture contents (MCs) (6%, 8%, and 11.5%) were subjected to four levels of mechanical stresses (0 mJ (i.e., control), 2 mJ, 4 mJ, and 6 mJ), followed by germination tests. Herein, we acquired hyperspectral images across visible to near-infrared (Vis-NIR) (450-1100 nm) and short-wave infrared (SWIR) (1000-2500 nm) ranges and used principal component analysis (PCA) for data exploration. Subsequently, mean spectra from the samples were used to develop partial least squares-discriminant analysis (PLS-DA) models utilizing key wavelengths to classify flaxseeds based on the extent of mechanical damage. The models developed using Vis-NIR and SWIR wavelengths demonstrated promising performance, achieving precision and recall rates >85% and overall accuracies of 90.70% and 93.18%, respectively. Partial least squares regression (PLSR) models were developed to predict germinability, resulting in R2-values of 0.78 and 0.82 for Vis-NIR and SWIR ranges, respectively. The study showed that HSI could be a potential alternative to conventional methods for fast, non-destructive, and reliable assessment of mechanical damage in flaxseeds.
Keywords: chemometrics; flaxseeds; hyperspectral imaging; mechanical damage; oilseed quality.