Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of oysters. The Jinjiang oyster ( Crassostrea ariakensis) is an economically and ecologically important species in China. In the present study, RNA sequencing (RNA-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-seq) were performed to investigate gene expression and chromatin accessibility variations in oysters with different glycogen contents. Analysis identified 9 483 differentially expressed genes (DEGs) and 7 215 genes with significantly differential chromatin accessibility (DCAGs) were obtained, with an overlap of 2 600 genes between them. Notably, a significant proportion of these genes were enriched in pathways related to glycogen metabolism, including "Glycogen metabolic process" and "Starch and sucrose metabolism". In addition, genome-wide association study (GWAS) identified 526 single nucleotide polymorphism (SNP) loci associated with glycogen content. These loci corresponded to 241 genes, 63 of which were categorized as both DEGs and DCAGs. This study enriches basic research data and provides insights into the molecular mechanisms underlying the regulation of glycogen metabolism in C. ariakensis.
糖原是水产贝类新陈代谢过程中最有效的能量储备物质，也与牡蛎的风味和品质密切相关。 近江牡蛎是我国重要的经济种和生态种，该研究通过对近江牡蛎转录组测序（RNA-seq）和转座酶可及染色质测序（ATAC-seq）的数据分析，探明了不同糖原含量牡蛎性腺组织的基因表达和染色质开放程度的变化情况。该研究共获得了9483个差异表达基因（DEG）和7215个染色质开放程度差异基因（DCAG），DEGs与DCAGs的交集有2600个。其中部分基因富集在与糖原代谢相关的通路中，例如“糖原代谢过程”和“淀粉和蔗糖代谢”。此外，该研究通过全基因组关联分析（GWAS）获得了526个与近江牡蛎糖原含量相关的SNP位点，这些位点与241个基因相关，这些基因中的63个基因属于DEGs和DCAGs。该研究将丰富基础研究数据，并为牡蛎糖原代谢调节的分子机制提供更多的见解。.
Keywords: ATAC; Crassostrea ariakensis; GWAS; Glycogen; Transcriptome.