Lipidomic insights into the reaction of baking lipases in cakes

Charlotte Dorothea Stemler; Sabrina Geisslitz; Adele Cutignano; Katharina Anne Scherf

doi:10.3389/fnut.2023.1290502

Lipidomic insights into the reaction of baking lipases in cakes

Front Nutr. 2023 Dec 12:10:1290502. doi: 10.3389/fnut.2023.1290502. eCollection 2023.

Authors

Charlotte Dorothea Stemler¹, Sabrina Geisslitz¹, Adele Cutignano², Katharina Anne Scherf¹

Affiliations

¹ Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
² Istituto di Chimica Biomolecolare (ICB), Consiglio Nazionale delle Ricerche (CNR), Pozzuoli (Napoli), Italy.

Abstract

Lipases are promising improvers of cake batter and baking properties. Their suitability for use in various cake formulations cannot be predicted yet, because the reactions that lead to macroscopic effects need to be unravelled. Therefore, the lipidome of three different cake recipes with and without lipase treatment was assessed by ultra high performance liquid chromatography-mass spectrometry before and after baking. By comparing the reaction patterns of seven different lipases in the recipes with known effects on texture, we show that lipase substrate specificity impacts baking quality. Key reactions for the recipes were identified with the help of principal component analysis. In the eggless basic cake, glyceroglycolipids are causal for baking improvement. In pound cake, lysoglycerophospholipids were linked to textural effects. Lipase substrate specificity was shown to be dependent on the recipe. Further research is needed to understand how recipes can be adjusted to achieve optimal lipase substrate specificity for desirable batter and baking properties.

Keywords: UHPLC-MS; baking; glyceroglycolipid; lipids; substrate specificity.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This IGF project of the FEI was supported via AiF within the program for promoting the Industrial Collective Research (IGF) of the German Ministry of Economics and Climate Action (BMWK), based on a resolution of the German Parliament. Project AiF 20771N. Measurements at the Q-Exactive Plus Orbitrap mass spectrometer were funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation). Project number 445432254. The research stay at the CNR was funded by the Karlsruhe House of Young Scientists (KHYS). We acknowledge support by the KIT-Publication Fund of the Karlsruhe Institute of Technology.