Comparison of the gut microbiota and metabolism in different regions of Red Swamp Crayfish (Procambarus clarkii)

Songyi Liu; Ziyan Wang; Ze Wang; Qiaoli Wu; Jun Zhou; Rixin Wang; Jiaojiao Han; Xiurong Su

doi:10.3389/fmicb.2023.1289634

Comparison of the gut microbiota and metabolism in different regions of Red Swamp Crayfish (Procambarus clarkii)

Front Microbiol. 2023 Dec 22:14:1289634. doi: 10.3389/fmicb.2023.1289634. eCollection 2023.

Authors

Songyi Liu^{1

2}, Ziyan Wang^{1

2}, Ze Wang^{1

2}, Qiaoli Wu^{1

2}, Jun Zhou^{1

2}, Rixin Wang^{1

2}, Jiaojiao Han^{1

2}, Xiurong Su^{1

2}

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo, China.
² School of Marine Science, Ningbo University, Ningbo, China.

Abstract

Background: The gut microbiota is very important for maintaining the homeostasis and health of crustaceans. Many factors affect the gut microbiota of crustaceans, one of which is temperature. However, it is currently unclear how temperature affects the gut microbiota and metabolites of Procambarus clarkii.

Methods: Using metagenomic sequencing and gas chromatography-mass spectrometry (GC-MS) techniques, the gut microbiota and metabolites of P. clarkii from Hubei (HB), Jiangsu (JS), Shandong (SD), and Zhejiang (ZJ) in China were investigated.

Results: Under the impact of temperature, the gut microbiota and metabolites of P. clarkii exhibit a specific trend of change. The primary pathogenic bacteria affecting P. clarkii are Citrobacter, Enterobacterium, and Aeromonas, which are affected by temperature. Two metabolites, namely, sugars and amino acids, are regulated by temperature.

Implication: This study demonstrated that the gut microbiota and gut metabolites of P. clarkii were considerably affected by temperature. It provides a theoretical basis for the systematic study of P. clarkii and provides a basis for a healthy culture of P. clarkii.

Keywords: Procambarus clarkii; dominant bacteria; intestinal microbiota; metabolites; metagenome.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was supported by the One Health Interdisciplinary Research Project, Ningbo University, the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University (ZS20190105), and KC Wong Magna Fund in Ningbo University.