Complete mitochondrial genome of Mastigias papua (Scyphozoa: Rhizostomeae: Mastigiidae) based on next-generation sequencing and phylogenetic analysis

Wangxiao Xia; Hui Jiang; Wenbo Fan; Xiaomin Li; Yaowen Liu; Lixian Xu; Xingchun Gou

doi:10.1080/23802359.2023.2281033

Complete mitochondrial genome of Mastigias papua (Scyphozoa: Rhizostomeae: Mastigiidae) based on next-generation sequencing and phylogenetic analysis

Mitochondrial DNA B Resour. 2023 Nov 27;8(11):1294-1297. doi: 10.1080/23802359.2023.2281033. eCollection 2023.

Authors

Wangxiao Xia^{1

2}, Hui Jiang³, Wenbo Fan², Xiaomin Li², Yaowen Liu⁴, Lixian Xu², Xingchun Gou¹

Affiliations

¹ Institute of Basic Translational Medicine, Shaanxi Key Laboratory of Brain Disorders, Xi'an Medical University, Xi'an, China.
² Department of Anesthesiology, School of Stomatology, State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Air Force Military Medical University, Xi'an, China.
³ National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, Zhoushan, China.
⁴ College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.

Abstract

We assembled the complete mitochondrial genome (mitogenome) of Mastigias papua (Scyphozoa: Rhizostomeae: Mastigiidae) by the data generated from the next-generation sequencing platform. The complete mitogenome of M. papua was 16,560 bp in length, containing 14 protein-coding genes, two transfer RNA genes, and two ribosomal RNA genes. The base compositions were A 30.65%, C 15.16%, G 16.34%, and T 37.86%, with a gene arrangement similar to the mitogenomes derived from other representatives of Scyphozoa. Based on the 13 common protein-coding genes of 16 species within Scyphozoa, we constructed the phylogenetic tree and found that M. papua has a close relationship with Cassiopea andromeda and Cassiopea xamachana. All these species belong to an order of jellyfish Rhizostomeae, which have similar morphological characteristics. This is agreement with the conclusion we got by the phylogenetic relationship analysis using molecular data. This research has practical implications for advancing understanding of the phylogenetic relationships, taxonomic classifications, and phylogeography within Scyphozoa.

Keywords: Mastigias papua; mitochondrial genome; next-generation sequencing; phylogenetic analysis.

Grants and funding

This research was funded by the National Natural Science Foundation of China [32100406], Chinese Academy of Sciences, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology [GREKF21-07], and Scientific Research Fund of Shaanxi Provincial Education Department [21JK0888].