Identification of neural circuits controlling male sexual behavior and sexual motivation by manganese-enhanced magnetic resonance imaging

Lorena Gaytán-Tocavén; Alejandro Aguilar-Moreno; Juan Ortiz; Sarael Alcauter; Edwards Antonio-Cabrera; Raúl G Paredes

doi:10.3389/fnbeh.2023.1301406

Identification of neural circuits controlling male sexual behavior and sexual motivation by manganese-enhanced magnetic resonance imaging

Front Behav Neurosci. 2023 Dec 21:17:1301406. doi: 10.3389/fnbeh.2023.1301406. eCollection 2023.

Authors

Lorena Gaytán-Tocavén¹, Alejandro Aguilar-Moreno², Juan Ortiz², Sarael Alcauter², Edwards Antonio-Cabrera², Raúl G Paredes^{1

2}

Affiliations

¹ Escuela Nacional de Estudios Superiores, Unidad Juriquilla, UNAM, Querétaro, Mexico.
² Instituto de Neurobiología, UNAM, Querétaro, Mexico.

Abstract

Introduction: Different techniques have been used to identify the brain regions that control sexual motivation and sexual behavior. However, the influence of sexual experience on the activation of these brain regions in the same subject is unknown. Using manganese-enhanced magnetic resonance imaging (MEMRI), we analyzed the activation of brain regions in the sexual incentive motivation (SIM) and the partner preference PP (tests) on weeks 1, 5, and 10 in male rats tested for 10 weeks. AIM. In experiment 1, we analyzed the possible toxic effects of 16 mg/kg of MnCl₂ on male sexual behavior, running wheel, and motor execution. In experiment 2, subjects were tested for SIM and PP using MEMRI.

Methods: In both experiments, a dose of 16 mg/kg (s.c) of chloride manganese (MnCl₂) was administered 24 h before subjects were tested and placed immediately thereafter in a 7-Tesla Bruker scanner.

Results: In experiment 1, the dose of 16 mg/kg of MnCl₂ did not induce behavioral alterations that could interfere with interpreting the imaging data. In experiment 2, we found a clear preference for the female in both the SIM and PP tests. We found a higher signal intensity in the olfactory bulb (OB) in week 1 of the SIM test compared to the control group. We also found increased signal intensity in the socio-sexual behavior and mesolimbic reward circuits in the SIM test in week 1. In the PP test, we found a higher signal intensity in the ventral tegmental area (VTA) in week 10 compared to the control group. In the same test, we found increased signal intensity in the socio-sexual and mesolimbic reward circuits in week 5 compared to the control group. Cohen's d analysis of the whole brain revealed that as the subjects gained sexual experience we observed a higher brain activation in the OB in the SIM group. The PP group showed higher brain activation in the cortex and subcortical structures as they acquired sexual experience.

Discussion: As the subjects gain sexual experience, more structures of the reward and socio-sexual circuits are recruited, resulting in different, and large brain activations.

Keywords: MEMRI; manganese; neural circuits; sexual behavior; sexual motivation.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. Grant DGAPA IN214524 supported this research. LG-T received a stipend from DGAPA.