The application of approaches in detecting ferroptosis

Zheyi Chen; Hongbing Lin; Xiaoyu Wang; Guiqi Li; Na Liu; Manli Zhang; Yuqin Shen

doi:10.1016/j.heliyon.2023.e23507

The application of approaches in detecting ferroptosis

Heliyon. 2023 Dec 13;10(1):e23507. doi: 10.1016/j.heliyon.2023.e23507. eCollection 2024 Jan 15.

Authors

Zheyi Chen¹, Hongbing Lin², Xiaoyu Wang¹, Guiqi Li¹, Na Liu¹, Manli Zhang¹, Yuqin Shen¹

Affiliations

¹ Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong 510182, China.
² Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, China.

Abstract

Ferroptosis is a regulatory cell death (RCD) caused by iron-dependent lipid peroxidation, which is the backbone of regulating various diseases such as tumor, nervous system diseases and so on. Despite ferroptosis without specific detection methods currently, there are numerous types of detection technology commonly used, including flow cytometry, cell activity assay, microscopic imaging, western blotting, quantitative polymerase chain reaction (qPCR). In addition, ferroptosis could be detected by quantifying oxygen-free radicals reactive oxygen species (ROS), the lipid metabolite (malondialdehyde ((MDA)), related pathways and observing mitochondrial damage. In the face of numerous detection methods, how to choose appropriate detection methods based on experimental purposes has become a problem that needs to be solved at present. In this review, we summarized the commonly used detection methods of the critical substances in the process of ferroptosis, in the hope of facilitating the comprehensive study of ferroptosis, with a view to providing a guidance for subsequent related research.

Keywords: Cell death; Detection methods; Ferroptosis.

Publication types

Review