Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters

Jihyun Hwang; Jiae Aum; Seong Jae Lee; Jeong Min Mun; Seung Wook Kim; Min-Yu Chung; In-Hwan Kim; Byung Hee Kim

doi:10.1007/s10068-023-01337-7

Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters

Food Sci Biotechnol. 2023 May 30;33(1):159-170. doi: 10.1007/s10068-023-01337-7. eCollection 2024 Jan.

Authors

Jihyun Hwang¹, Jiae Aum¹, Seong Jae Lee², Jeong Min Mun², Seung Wook Kim², Min-Yu Chung³, In-Hwan Kim⁴, Byung Hee Kim¹

Affiliations

¹ Department of Food and Nutrition, Sookmyung Women's University, Seoul, 04310 Korea.
² Research Center, Ottogi Corporation, Gyeonggi, 14060 Korea.
³ Department of Food and Nutrition, Gangseo University, Seoul, 07661 Korea.
⁴ Department of Food and Nutrition, Korea University, Seoul, 02841 Korea.

PMID: 38186626
PMCID: PMC10767010 (available on 2024-05-30)
DOI: 10.1007/s10068-023-01337-7

Abstract

Candida antarctica lipase B (CALB) is regarded as non-regiospecific. This study aimed to investigate the regiospecificity of CALB in the solvent-free interesterification of high-oleic sunflower oil with stearic acid ethyl ester for 1,3-distearoyl-2-oleoylglycerol (SOS)-rich fat preparation using a packed bed reactor. The content ratio of 1,2-distearoyl-3-oleoylglycerol (SSO) to SOS (denoted by SSO/SOS content) obtained using Lipozyme 435 (a commercially immobilized CALB; 0-4.1%), at residence times (1-32 min) was similar to that obtained using Lipozyme RM IM (0-3.0%), but lower than that obtained using Lipozyme TL IM (6.0-39.4%). When immobilized on Lewatit VP OC 1600, Lipozyme CALB had an SSO/SOS content of 0-10.4%, which was greater than that of Palatase 20,000 L (0-1.1%) but was lower than that of Lipozyme TL 100 L (8.8-97.7%). Our findings suggest that immobilized CALB shows distinct sn-1,3 regiospecificity in the interesterification of triacylglycerol with fatty acid ethyl esters.

Keywords: Candida antarctica Lipase B; Immobilization; Interesterification; Lipozyme 435; Sn-1,3 specificity.

© The Korean Society of Food Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.