Complete genome sequence of Acinetobacter indicus and identification of the hydrolases provides direct insights into phthalate ester degradation

Huiqin Huang; Youqiang Xu; Mengwei Lin; Xiuting Li; Hua Zhu; Kun Wang; Baoguo Sun

doi:10.1007/s10068-023-01334-w

Complete genome sequence of Acinetobacter indicus and identification of the hydrolases provides direct insights into phthalate ester degradation

Food Sci Biotechnol. 2023 May 22;33(1):103-113. doi: 10.1007/s10068-023-01334-w. eCollection 2024 Jan.

Authors

Huiqin Huang^#¹, Youqiang Xu^#¹, Mengwei Lin¹, Xiuting Li^{1

2}, Hua Zhu^{2

3}, Kun Wang^{2

3}, Baoguo Sun^{1

2

4}

Affiliations

¹ School of Food and Human Health, Beijing Technology and Business University, No. 33, Fucheng Road, Haidian District, Beijing, 100048 China.
² Key Laboratory of Brewing Microbiome and Enzymatic Molecular Engineering, China General Chamber of Commerce, Beijing, 100048 China.
³ Beijing Huadu Wine Food Limited Liability Company, Beijing, 102212 China.
⁴ Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing, 100048 China.

^# Contributed equally.

Abstract

A strain designated Acinetobacter indicus WMB-7 with the ability to hydrolyze phthalate esters (PAEs) was isolated from the fermented grains of Baijiu. The genome of the strain was sequenced with a length of 3,256,420 bp and annotated with 3183 genes, of which 36 hydrolases encoding genes were identified. The hydrolases were analyzed by protein structure modeling and molecular docking, and 14 enzymes were docked to the ligand di-butyl phthalate with the catalytic active regions, and showed binding affinity. The 14 enzymes were expressed in E. coli and 5 of them showed the ability for PAEs hydrolysis. Enzyme GK020_RS15665 showed high efficiency for PAEs hydrolysis and could efficiently hydrolyze di-butyl phthalate under an initial concentration of 1000 mg/L with a half-life of 4.24 h. This work combined a series of methods for identifying PAEs hydrolases and offered a molecular basis for PAEs degradation of A. indicus strains from Baijiu.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-023-01334-w.

Keywords: Acinetobacter indicus; Baijiu; Ester bond hydrolysis; Molecular docking; Phthalate esters.

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