Construction and Application of a Pepsin-Functionalized Covalent Organic Framework with Prominent Chiral Recognition Ability

Jiale Zhang; Xue Liu; Qixuan Mu; Ruijun Li; Yibing Ji

doi:10.1002/chem.202303827

Construction and Application of a Pepsin-Functionalized Covalent Organic Framework with Prominent Chiral Recognition Ability

Chemistry. 2024 Mar 12;30(15):e202303827. doi: 10.1002/chem.202303827. Epub 2024 Jan 22.

Authors

Jiale Zhang^{1

2}, Xue Liu^{1

2}, Qixuan Mu^{1

2}, Ruijun Li^{1

2}, Yibing Ji^{1

2}

Affiliations

¹ Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, China.
² Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing, 210009, China.

PMID: 38183168
DOI: 10.1002/chem.202303827

Abstract

The stable Pepsin@covalent organic framework (Pepsin@COF) were constructed base on matching COF pore diameter to pepsin dimension. It exhibits excellent chiral recognition capabilities (e. e. % up to 62.63 %) and potential for enantioseparation. Furthermore, a positive correlation between the immobilized enzyme activity and chiral recognition was revealed, offering insights for the design of biocatalytic nanosystems in chiral separation.

Keywords: covalent organic framework; enantioselective recognition; enantioseparation; enzymatic activity; pepsin.